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Quantifying contribution of SOS1 and GORK plasma membrane transporters towards salinity tolerance in barley by the MIFE technology

Citation

Wu, H and Shabala, SN and Zhou, M and Shabala, L, Quantifying contribution of SOS1 and GORK plasma membrane transporters towards salinity tolerance in barley by the MIFE technology, The 16th Australian Barley Technical Symposium, 8-11 September 2013, Melbourne, Australia (2013) [Conference Extract]

Abstract

The relative contribution of SOS1 and GORK plasma membrane transporters towards salinity \tolerance in barley was quantified using the MIFE (microelectrode ion flux estimation) technique to screen 50 barley varieties for their ability to exclude sodium from uptake (e.g. the functional activity of SOS1 Na+/H+ exchanger) and to retain potassium in leaf mesophyll (functional activity of GORK channels). It was found that salt-sensitive varieties Gairdner, Naso Nijo, and ZUG403 showed relatively low (around -200 nmol m-2 s-1) rate of Na+ pumping, while salt-tolerant varieties ZUG293, Yerong and CPI showed 2.5 to 3 fold (the range of -500 to -600 nmol m-2 s-1) higher SOS1 Na+/H+ activity. Also, a 5-fold variability in the magnitude of net K+ efflux was found among barley accessions (flux range from -300 to -1600 nmol m-2 s-1). Minimal K+ leak (hence, the highest tissue tolerance) was measured in varieties CM72, Numar, YYXT and Yerong; all known to possess superior salinity stress tolerance. It is suggested that (1) salinity tolerance in barley is conferred by multiple physiological mechanisms operating via a range of plasma membrane and organelle transporters; (2) the MIFE technique may be used as an efficient screening tool to quantify the functional activity of key membrane transporters contributing to above traits. Furthermore, the MIFE technique may also be suitable for screening DH population for mapping genes conferring key physiological traits behind salinity tolerance in plants.

Item Details

Item Type:Conference Extract
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Wu, H (Mr Honghong Wu)
Author:Shabala, SN (Professor Sergey Shabala)
Author:Zhou, M (Professor Meixue Zhou)
Author:Shabala, L (Dr Lana Shabala)
ID Code:85564
Year Published:2013
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-07-18
Last Modified:2014-04-29
Downloads:0

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