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The effects of dietary vitamin A in rotifers on the performance and skeletal abnormality of striped trumpeter Latris lineata larvae and post larvae

Citation

Negm, RK and Cobcroft, JM and Brown, MR and Nowak, BF and Battaglene, SC, The effects of dietary vitamin A in rotifers on the performance and skeletal abnormality of striped trumpeter Latris lineata larvae and post larvae, Aquaculture, 404-405 pp. 105-115. ISSN 0044-8486 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier.

DOI: doi:10.1016/j.aquaculture.2013.04.018

Abstract

Several nutritional studies have linked dietary vitamin A (VA) to skeletogenesis in marine fish larvae. In our study, rotifers were enriched with eight levels of retinyl palmitate. Striped trumpeter (Latris lineata) larvae were fed rotifers twice daily, VA-enriched rotifers (morning feed) and Algamac−3050 enriched rotifers (afternoon feed), in greenwater systems from6 to 18 days post-hatch (dph). The VA-enriched rotifers had incorporated 0, 6, 14, 26, 57, 109, 215 and 388 ng retinyl palmitate mg−1 dryweight (DW) following 2 h enrichment with emulsions containing 0 (control), 68, 187, 532, 1402, 2670, 4808 and 9523 ng retinyl palmitate mg−1 emulsion. After the rotifer feeding phase (6 to 18 dph), the larvae were fed Algamac−3050 enriched Artemia until 43 dph. The pattern of increasing VA in the enriched rotifers was not reflected in the larvae. Larvae incorporated 11.08 ± 0.27 ng total retinol mg−1 DW (mean ± SD) when fed rotifers containing 0.93 to 2.32 ng total retinol mg−1 DW (6 to 14 ng retinyl palmitate mg−1 DW) and incorporated a lower level of 5.57 ± 0.30 ng total retinol mg−1 DW when fed rotifers with 0.52 (control) or ≥16.31 ng total retinol mg−1 DW (0 and ≥57 ng retinyl palmitate mg−1 DW). By 43 dph, neither larval growth in length (16.0 ± 0.1 mm) or dry weight (4.57 ± 0.20 mg), nor survival (34.8 ± 10.6%), were significantly affected by increasing dietary levels of retinyl palmitate. The prevalence of vertebral column malformations in 43 dph post larvae was positively correlated with total retinol content of larvae at 16 dph (R2 = 0.55, P b 0.001). Unlike other studies on a range of marine fish species, retinyl palmitate enrichment in rotifers did not affect the type or severity of jaw malformations. By 43 dph, 81 ± 9% of post larvae displayed severe jaw malformations. Vitamin A daily inclusion of more than 123 ng total VA mg−1 DW rotifer, equivalent to more than 35 ng total retinol mg−1 DW rotifer (109 ng retinyl palmitate mg−1 DW rotifers), is recommended to reduce vertebral column malformations when L. lineata larvae are reared in greenwater.

Item Details

Item Type:Refereed Article
Keywords:arine fish Larvae, vitamin A (retinol), rotifers, dose-response, malformations
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Fin Fish (excl. Tuna)
Author:Negm, RK (Mrs Reham Negm)
Author:Cobcroft, JM (Dr Jennifer Cobcroft)
Author:Nowak, BF (Professor Barbara Nowak)
Author:Battaglene, SC (Associate Professor Stephen Battaglene)
ID Code:85510
Year Published:2013
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2013-07-12
Last Modified:2017-11-03
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