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The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley

Citation

Zhou, G and Delhaize, E and Zhou, M and Ryan, PR, The barley MATE gene, HvAACT1, increases citrate efflux and Al3+ tolerance when expressed in wheat and barley, Annals of Botany, 112, (3) pp. 603-612. ISSN 0305-7364 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 the authors.

DOI: doi:10.1093/aob/mct135

Abstract

Background and Aims Aluminium is toxic in acid soils because the soluble Al3+ inhibits root growth. A mechanism of Al3+ tolerance discovered in many plant species involves the release of organic anions from root apices. The Al3+-activated release of citrate from the root apices of Al3+-tolerant genotypes of barley is controlled by a MATE gene named HvAACT1 that encodes a citrate transport protein located on the plasma membrane. The aim of this study was to investigate whether expressing HvAACT1 with a constitutive promoter in barley and wheat can increase citrate efflux and Al3+ tolerance of these important cereal species.

Methods: HvAACT1 was over-expressed in wheat (Triticum aestivum) and barley (Hordeum vulgare) using the maize ubiquitin promoter. Root apices of transgenic and control lines were analysed for HvAACT1 expression and organic acid efflux. The Al3+ tolerance of transgenic and control lines was assessed in both hydroponic solution and acid soil.

Key Results and Conclusions: Increased HvAACT1 expression in both cereal species was associated with increased citrate efflux from root apices and enhanced Al3+ tolerance, thus demonstrating that biotechnology can complement traditional breeding practices to increase the Al3+ tolerance of important crop plants.

Item Details

Item Type:Refereed Article
Keywords:acid soil, aluminium, resistance, tolerance, wheat, transgenic, barley, Hordeum vulgare, Triticum aestivum
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Improvement (Selection and Breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Zhou, G (Mr Gaofeng Zhou)
Author:Zhou, M (Professor Meixue Zhou)
ID Code:85428
Year Published:2013
Web of Science® Times Cited:32
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-07-04
Last Modified:2015-02-04
Downloads:0

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