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Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)

Citation

Bian, M and Waters, I and Broughton, S and Zhang, X-Q and Zhou, M and Lance, R and Sun, D and Li, C, Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.), Molecular Breeding, 32, (1) pp. 155-164. ISSN 1380-3743 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s11032-013-9859-3

Abstract

Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals-introduced from CYMMIT (International Maize and Wheat Improvement Center)-was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion-deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.

Item Details

Item Type:Refereed Article
Keywords:QTL, aluminium tolerance, gene-specific markers, barley
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Biochemistry and Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
Author:Bian, M (Ms Miao Bian)
Author:Zhou, M (Professor Meixue Zhou)
ID Code:85407
Year Published:2013
Web of Science® Times Cited:7
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-07-04
Last Modified:2017-10-31
Downloads:0

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