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Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice


Lim, WLF and Lam, SM and Shui, G and Mondal, A and Ong, D and Duan, X and Creegan, R and Martins, IJ and Sharman, MJ and Taddei, K and Verdile, G and Wenk, MR and Martins, RN, Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice, Neurobiology of Aging: age-related phenomena, neurodegeneration and neuropathology, 34, (9) pp. 2217-2224. ISSN 0197-4580 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier

DOI: doi:10.1016/j.neurobiolaging.2013.03.012


Apolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer's disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE ɛ3 and ɛ4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE ɛ4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species.

Item Details

Item Type:Refereed Article
Keywords:APOE KI mice, high fat, high cholesterol, mass spectrometry, glycerophospholipids, oxysterols
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurosciences not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Sharman, MJ (Dr Matt Sharman)
ID Code:85350
Year Published:2013
Web of Science® Times Cited:14
Deposited By:Health Sciences A
Deposited On:2013-07-01
Last Modified:2017-11-07

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