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Effects of a high-fat, high-cholesterol diet on brain lipid profiles in apolipoprotein E ε3 and ε4 knock-in mice
journal contribution
posted on 2023-05-17, 18:36 authored by Lim, WLF, Lam, SM, Shui, G, Mondal, A, Ong, D, Duan, X, Creegan, R, Martins, IJ, Matthew SharmanMatthew Sharman, Taddei, K, Verdile, G, Wenk, MR, Martins, RNApolipoprotein E (ApoE) is important in facilitating the transport of lipids (cholesterol, phospholipids, and sulfatides) and plays a fundamental role in normal lipid metabolism. High cholesterol levels increases the risk of developing Alzheimer's disease. In this study, we investigated the effects of a high-fat high cholesterol (HFHC) diet on brain lipid profiles in 95 young and aged APOE ɛ3 and ɛ4 knock-in mice to determine whether diet leads to altered brain levels of a number of glycerophospholipids, sphingolipids, cholesterol precursors, cholesterol, cholesterol oxidation products, and cholesterol esters. The results in this study revealed significant changes in lipid levels. The HFHC-enriched diet influenced the levels of cholesterol esters. A sharp increase in cholesterol ester levels, particularly in the aged APOE ɛ4 diet-enriched group, might be suggestive of abnormal acyl-coenzyme A:cholesterol acyltransferase 1 (ACAT) activity and/or levels. Age exerts appreciable effects on the brain lipidome, especially with regard to polar lipid species.
History
Publication title
Neurobiology of Aging: age-related phenomena, neurodegeneration and neuropathologyVolume
34Issue
9Pagination
2217-2224ISSN
0197-4580Department/School
School of Health SciencesPublisher
Elsevier Inc.Place of publication
360 Park Ave South, New York, USA, Ny, 10010-1710Rights statement
Copyright 2013 ElsevierRepository Status
- Restricted