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Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae)

Citation

Smith, TM and York, PH and Stanley, AM and Macreadie, PI and Keough, MJ and Ross, DJ and Sherman, CDH, Microsatellite primer development for the seagrass Zostera nigricaulis (Zosteraceae), Conservation Genetic Resources, 5, (3) pp. 607-610. ISSN 1877-7252 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Springer Science+Business Media Dordrecht

DOI: doi:10.1007/s12686-013-9862-3

Abstract

Seagrasses are marine angiosperms with a worldwide distribution that form conspicuous beds in nearshore habitats. Despite being universally recognised as a foundation species that performs a number of important ecosystems functions (incl. sediment stabilisation, facilitation of biodiversity, nutrient cycling and carbon sequestration), global seagrass habitats are in decline. Resilience—the ability to recover from disturbance without switching to an alternative state—is paramount to the maintenance and persistence of seagrass habitats. Genetic diversity is a key component of seagrass resilience and contributes to an understanding of population structure, connectivity between populations, and reproductive strategies. Microsatellite primers were developed to investigate the resilience of the seagrass Zostera nigricaulis, which dominates subtidal habitats in the bays of south-eastern Australia.Wealso tested for cross-amplification of markers between Z. nigricaulis and previously developed markers for the sympatric species Z. muelleri to assess their applicability for use in assessing patterns of genetic diversity, population structure, and mating system. Using next-generation sequencing we isolated 11 novel microsatellite loci for Z. nigricaulis, 8 of which were polymorphic for the samples tested. Allelic diversity ranged from 1 to 8. None of the primer pairs developed for Z. nigricaulis cross-amplified in Z. muelleri; but 14 of 24 primer pairs previously developed for Z. muelleri amplified clearly in Z. nigricaulis samples with six of these showing polymorphism. The results demonstrate the applicability of the Z. nigricaulis microsatellite primers for use in the study of population genetics and limited cross-amplification with Z. muelleri.

Item Details

Item Type:Refereed Article
Keywords:Zostera nigricaulis  Microsatellite markers Cross-amplification  Genetic structure  Seagrass
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquatic Ecosystem Studies and Stock Assessment
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Coastal and Estuarine Environments
Author:Ross, DJ (Dr Jeff Ross)
ID Code:85317
Year Published:2013
Web of Science® Times Cited:6
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2013-06-27
Last Modified:2017-10-31
Downloads:0

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