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Pseudomonas aeruginosa uses multiple pathways to acquire iron during chronic infection in cystic fibrosis lungs
Citation
Konings, AF and Martin, LW and Sharples, MJ and Roddam, LF and Latham, R and Reid, DW and Lamont, IL, Pseudomonas aeruginosa uses multiple pathways to acquire iron during chronic infection in cystic fibrosis lungs, Infection and Immunity, 81, (8) pp. 2697-2704. ISSN 0019-9567 (2013) [Refereed Article]
Copyright Statement
Copyright 2013 American Society for Microbiology
Abstract
Pseudomonas aeruginosa chronically infects the lungs of more than 80% of adult patients with cystic fibrosis (CF) and is a major contributor to progression of disease pathology. P. aeruginosa requires iron for growth and has multiple iron uptake systems that have been studied in bacteria grown in laboratory culture. The purpose of this research was to determine which of these are active during infection in CF. RNA was extracted from 149 sputum samples obtained from 23 CF patients. Reverse transcription-quantitative real time PCR (RT-qPCR) was used to measure expression of P. aeruginosa genes encoding transport systems for the siderophores pyoverdine and pyochelin, for heme, and for ferrous ions. Expression of P. aeruginosa genes could be quantified in 89% of the sputum samples. Expression of genes associated with siderophore-mediated iron uptake was detected in most samples but was at low levels in some samples, indicating that other iron uptake mechanisms are active. Expression of genes encoding heme transport systems was also detected in most samples indicating that heme uptake occurs during infection in CF. Expression of feoB was detected in all sputum samples, implying an important role for ferrous ion uptake by P. aeruginosa in CF. Our data show that multiple P. aeruginosa iron uptake mechanisms are active in chronic CF infection and that RT-qPCR of RNA extracted from sputum provides a powerful tool for investigating bacterial physiology during infection in CF.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | cystic fibrosis, Pseudomonas, iron uptake |
| Research Division: | Medical and Health Sciences |
| Research Group: | Medical Microbiology |
| Research Field: | Medical Bacteriology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Respiratory System and Diseases (incl. Asthma) |
| Author: | Roddam, LF (Dr Louise Roddam) |
| Author: | Latham, R (Mr Roger Latham) |
| Author: | Reid, DW (Dr David Reid) |
| ID Code: | 85071 |
| Year Published: | 2013 |
| Funding Support: | National Health and Medical Research Council (544915) |
| Web of Science® Times Cited: | 45 |
| Deposited By: | Medicine (Discipline) |
| Deposited On: | 2013-06-13 |
| Last Modified: | 2014-05-07 |
| Downloads: | 0 |
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