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Accelerated Extraction for determination of Polycyclic Aromatic Hydrocarbons in Marine Biota

Citation

Sanz, J and Bartolome, L and Zuloaga, O and Gonzalez, L and Dietz, C and Camara, C, Accelerated Extraction for determination of Polycyclic Aromatic Hydrocarbons in Marine Biota, Analytical and Bioanalytical Chemistry, 384, (6) pp. 1331- 1340. ISSN 1618-2642 (2006) [Refereed Article]

Copyright Statement

Copyright 2006 Springer-Verlag

DOI: doi:10.1007/s00216-005-0249-5

Abstract

A rapid and simple method is proposed for determination of polycyclic aromatic hydrocarbons (PAH) in complex matrices such as marine biota. The method uses sonication, bymeans of an ultrasonic probe, as a new tool for assisted extraction, coupled with reversed-phase liquid chromatography (RP-LC) with fluorescence detection (FL) for determination of 16 US EPA priority PAH. Separation and detection of the 16 PAHwere complete in 45 min by RPLC with a C18 column and acetonitrile–water gradient elution. Multivariate optimisation of the variables affecting extraction (ultrasound radiation amplitude, sonication time, and temperature of the water-bath in which the extraction cell was placed) was conducted. The accuracy of the method was determined by analysis of a certified reference material and comparison of the results obtained with those from another method (microwave-assisted extraction and GC– MS). The new technique avoids the main problems encountered in the determination of PAH in complex matrices such as marine biota, and no clean-up step is necessary. The method was applied to determination of PAH in estuarine biota samples from the Urdaibai estuary (Biscay, Spain).

Item Details

Item Type:Refereed Article
Keywords:PAH, accelerated extraction
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Chemistry not elsewhere classified
Objective Division:Environment
Objective Group:Ecosystem Assessment and Management
Objective Field:Ecosystem Assessment and Management of Coastal and Estuarine Environments
Author:Dietz, C (Dr Christian Dietz)
ID Code:84834
Year Published:2006
Web of Science® Times Cited:34
Deposited By:Central Science Laboratory
Deposited On:2013-06-03
Last Modified:2013-07-03
Downloads:0

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