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Volatile organo-selenium speciation in biological matter by solid phase microextraction - moderate temperature multicapillary gas chromatography with microwave induced plasma atomic emission spectrometry detection

Citation

Dietz, C and Sanz, J and Ximenez, P and Madrid, Y and Camara, C, Volatile organo-selenium speciation in biological matter by solid phase microextraction - moderate temperature multicapillary gas chromatography with microwave induced plasma atomic emission spectrometry detection, Analytica Chimica Acta: International Journal Devoted to All Branches of Analytical Chemistry, 501 pp. 157-167. ISSN 0003-2670 (2004) [Refereed Article]

Copyright Statement

Copyright 2003 Elsevier B.V.

DOI: doi:10.1016/j.aca.2003.09.027

Abstract

Microwave induced plasma atomic emission spectrometry (MIP-AES) in combination with multicapillary (MC) gas chromatography could be proven to be useful for element specific detection of volatile species. Solid phase microextraction (SPME)was used for preconcentration and sample-matrix separation. The fiber desorption unit as well as the heating control for theMCcolumn were in-house developed and multicapillary column was operated at moderate temperatures (30100 ◦C). The method was optimized for organo-selenium species (dimethylselenide (DMSe), diethylselenide (DEtSe) and dimethyldiselenide (DMDSe)), using a chemometric approach. Stationary phases for the separation column were optimized using a conventional GC and contrasted with the results obtained with the MC. Application was focussed on selenium accumulating biological matter, such as lupine, yeast, Indian mustard and garlic. These samples were grown in hydroponic solution containing inorganic selenium (Na2SeO3 and Na2SeO4). SPME sampling was carried out in fixed volume flow boxes in headspace above the living plants and in vials using treated samples. Results demonstrate inorganic selenium transformation into volatile organic species during metabolism. Separation is fast, a chromatogram can be obtained in less than 3 min and detection limits were at sub-ppb level for all investigated species. The system is independent from the use of a conventional gas chromatographic oven and can be used as a versatile alternative to highly cost intensive methods such as GCICP-MS.

Item Details

Item Type:Refereed Article
Keywords:MIP, SPME, selenium speciaTION
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Analytical Chemistry not elsewhere classified
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural Crops
Objective Field:Horticultural Crops not elsewhere classified
Author:Dietz, C (Dr Christian Dietz)
ID Code:84828
Year Published:2004
Web of Science® Times Cited:52
Deposited By:Central Science Laboratory
Deposited On:2013-06-03
Last Modified:2013-07-02
Downloads:0

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