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SPME-multicapillary GC coupled to different detection systems applied to volatile organo-selenium speciation in yeast

Citation

Dietz, C and Landaluze, JS and Ximenez-Embun, P and Madrid-Albarran, Y and Camara, C, SPME-multicapillary GC coupled to different detection systems applied to volatile organo-selenium speciation in yeast, Journal of Analytical Atomic Spectrometry, 19, (2) pp. 260-266. ISSN 0267-9477 (2004) [Refereed Article]

Copyright Statement

Copyright 2004 The Royal Society of Chemistry.

DOI: doi:10.1039/b308544a

Abstract

In this work the versatility of solid phase microextraction (SPME) in combination with multicapillary gas chromatography (MC–GC) was evaluated using different common detectors for organo-selenium speciation. The methods compared for detection were inductively coupled plasma mass spectrometry (ICP–MS), microwave induced plasma atomic emission spectroscopy (MIP–AES) and atomic fluorescence spectroscopy (AFS). All detectors were found to be suitable, with highest sensitivity being obtained for MIP–AES detection, with detection limits of 0.57 ng ml−1 for dimethyl selenide, 0.47 ng ml−1 for diethyl selenide and 0.19 ng ml−1 for dimethyl diselenide. The method was applied to the determination of volatile alkyl selenides in selenium enriched yeast samples, which revealed that the presence of inorganic selenium gives rise to at least seven different volatile species after metabolization, with dimethyl diselenide the predominant species. Commercial pasteurized yeast, containing mainly selenomethionine for its use as a food supplement, was found to be still active and produces considerable amounts of organoselenium compounds.

Item Details

Item Type:Refereed Article
Keywords:SPME, yeast, multicapillary GC
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
Author:Dietz, C (Dr Christian Dietz)
ID Code:84825
Year Published:2004
Web of Science® Times Cited:37
Deposited By:Central Science Laboratory
Deposited On:2013-06-03
Last Modified:2017-06-06
Downloads:0

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