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Low-pH and aluminum resistance in Arabidopsis correlates with high cytosolic magnesium content and increased magnesium uptake by plant roots

Citation

Bose, J and Babourina, O and Shabala, S and Rengel, Z, Low-pH and aluminum resistance in Arabidopsis correlates with high cytosolic magnesium content and increased magnesium uptake by plant roots, Plant and Cell Physiology, 54, (7) pp. 1093-1104. ISSN 0032-0781 (2013) [Refereed Article]

Copyright Statement

copyright 2013 The Authors

DOI: doi:10.1093/pcp/pct064

Abstract

Low-pH stress and Al3+ toxicity affect root growth in acid soils. It was hypothesized that the capacity of genotypes to maintain Mg2+ uptake in acidic environments may contribute to low-pH and Al resistance, but explicit evidence is lacking. In this work, an Al-resistant alr104 mutant and two Al-sensitive mutants (als5 and als3) of Arabidopsis thaliana were compared with the wild type (Col-0) for Mg2+ uptake and intracellular Mg2+ concentration under low-pH and combined low-pH/Al stresses. Magnesium accumulation in roots was measured in long-term (7 d) experiments. The Mg2+ fluxes were measured using ion-sensitive microelectrodes at the distal elongation and the mature root zones in short-term (060 min) experiments. Intracellular Mg2+ concentrations were measured in intact root cells at the distal elongation zone using magnesium-specific fluorescent dye and fluorescent lifetime imaging (FLIM) analysis. Under low-pH stress, Arabidopsis mutants als5 and alr104 maintained a higher Mg concentration in roots, and had greater Mg2+ influx than the wild type and the als3 mutant. Under combined low-pH/Al treatment, Al-resistant genotypes (wild type and alr104) maintained a higher Mg2+ accumulation, and had a higher Mg2+ influx and higher intracellular Mg2+ concentration than Al-sensitive genotypes (als3 and als5). Overall, these results show that increased Mg2+ uptake correlates with an enhanced capacity of Arabidopsis genotypes to cope with low-pH and combined low-pH/Al stresses.

Item Details

Item Type:Refereed Article
Keywords:aluminum toxicity, Arabidopsis thaliana, intracellular magnesium, low pH, magnesium flux
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Agricultural and Veterinary Sciences
Author:Bose, J (Dr Jayakumar Bose)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:84818
Year Published:2013
Web of Science® Times Cited:22
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-06-03
Last Modified:2017-01-11
Downloads:0

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