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Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells

Citation

Zulfakar, SS and White, JD and Ross, T and Tamplin, ML, Cultured C2C12 cell lines as a model for assessment of bacterial attachment to bovine primary muscle cells, Meat Science, 94, (2) pp. 215-219. ISSN 0309-1740 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier

DOI: doi:10.1016/j.meatsci.2013.01.017

Abstract

The mechanisms of bacterial attachment to meat tissues need to be understood to enhance meat safety interventions. However, little is known about attachment of foodborne pathogens to meat muscle cells. In this study, attachment of six Escherichia coli and two Salmonella strains to primary bovine muscle cells and a cultured muscle cell line, C2C12, was measured, including the effect of temperature. At 37 C, all but one strain (EC623) attached to C2C12 cells, whereas only five of eight strains (M23Sr, H10407, EC473, Sal1729a and Sal691) attached to primary cells. At 10 C, two strains (H10407 and EC473) attached to C2C12 cells, compared to four strains (M23Sr, EC614, H10407 and Sal1729a) of primary cells. Comparing all strains at both temperatures, EC614 displayed the highest CFU per C2C12cell (4.60 2.02 CFU/muscle cell at 37 C), whereas greater numbers of M23Sr attached per primary cell (51.88 39.43CFU/muscle cell at 37 C). This study indicates that primary bovine muscle cells may provide a more relevant model system to study bacterial attachment to beef carcasses compared to cell lines such as C2C12.

Item Details

Item Type:Refereed Article
Keywords:attachment, E. coli, Salmonella, C2C12, primary bovine muscle cells
Research Division:Engineering
Research Group:Food Sciences
Research Field:Food Packaging, Preservation and Safety
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Food Safety
Author:Zulfakar, SS (Ms Sarah Zulfakar)
Author:White, JD (Dr Jason White)
Author:Ross, T (Associate Professor Tom Ross)
Author:Tamplin, ML (Professor Mark Tamplin)
ID Code:84796
Year Published:2013
Web of Science® Times Cited:2
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-06-03
Last Modified:2015-02-02
Downloads:0

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