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Marine microheterotrophs thraustochytrids are emerging as a potential source for commercial production of polyunsaturated fatty acids (PUFA) that have nutritional and pharmacological values. With prospective demand for PUFAs increasing, biotechnological companies are looking for potential increases in those valuable products. However, high levels of NaCl in the culture media required for optimal thraustochytrid growth and PUFA production poses a significant problem to the biotechnological industry due to corrosion of fermenters calling for a need to reduce the amount of NaCl in the culture media, without imposing penalties on growth and yield of cultured organisms. Earlier, as reported by Shabala et al. (Environ Microbiol 11:1835–1843, 2009), we have shown that thraustochytrids use sodium predominantly for osmotic adjustment purposes and, as such, can be grown in low-salt environment without growth penalties, providing the media osmolality is adjusted. In this study, we verify if that conclusion, made for one specific strain and osmolyte only, is applicable to the larger number of strains and organic osmotica, as well as address the issue of yield quality (e.g., PUFA production in low-saline media). Using mannitol and sucrose for osmotic adjustment of the growth media enabled us to reduce NaCl concentration down to 1 mM; this is 15–100-fold lower than any method proposed so far. At the same time, the yield of essential PUFAs was increased by 15 to 20 %. Taken together, these results suggest that the proposed method can be used in industrial fermenters for commercial PUFA production.

Item Type:	Refereed Article
Keywords:	thraustochytrids, biotechnology, fermenter corrosion, osmotic adjustment, compatible solutes, polyunsaturated fatty acids (PUFA)
Research Division:	Biological Sciences
Research Group:	Microbiology
Research Field:	Microbial Ecology
Objective Division:	Health
Objective Group:	Public Health (excl. Specific Population Health)
Objective Field:	Food Safety
UTAS Author:	Shabala, L (Associate Professor Lana Shabala)
UTAS Author:	McMeekin, T (Professor Thomas McMeekin)
UTAS Author:	Shabala, S (Professor Sergey Shabala)
ID Code:	84753
Year Published:	2013
Web of Science® Times Cited:	7
Deposited By:	Tasmanian Institute of Agriculture
Deposited On:	2013-05-30
Last Modified:	2014-04-04
Downloads:	1 View Download Statistics