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Direct determination of transition metals in mussel tissue digests using high-performance chelation ion chromatography with monolithic silica based chelating ion exchangers

Citation

McGillicuddy, N and Nesterenko, EP and Jones, P and Caldarola, D and Onida, B and Townsend, AT and Mitev, DP and Nesterenko, PN and Paull, B, Direct determination of transition metals in mussel tissue digests using high-performance chelation ion chromatography with monolithic silica based chelating ion exchangers, Analytical Methods, 5, (11) pp. 2666-2673. ISSN 1759-9660 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Royal Society of Chemistry

DOI: doi:10.1039/C3AY40301G

Abstract

A new high performance chelation ion chromatography method was developed for the direct separation and detection of selected transition metals in shellfish (mussel) tissue digest samples. Using two different bonded silica monolithic chelating stationary phases, exhibiting differing selectivities for metals of interest, were applied within the method. The bonded chelating chemistries were N-hydroxyethyliminodiacetic (HEIDA) and N-(2-hydroxyethyl)-N-(2-[phosphonomethyl)amino]acetic (HEPMA) acids. Quantitative determination of Mn(II), Cd(II) and Zn(II) concentrations within shellfish tissue (Mytilus edulis), following microwave assisted acid digestion. Post-column detection was achieved using spectophotometric detection at 510 nm after reaction with 4-(2-pyridylazo) resorcinol. The eluents employed were 0.5 M KNO3, pH 2.4, for the HEIDA-bonded stationary phase, and 0.1 M KNO3, pH 2.6, for the HEPMA-bonded monolithic column. The HEPMA phase provided improved resolution between Mn(II) and interfering alkaline earth metal cations compared to the HEIDA-bonded phase. Concentrations of metals were determined using standard addition, and Cd(II), Mn(II), and Zn(II) cations were detected at <10 μg g−1, <50 μg g−1 and <700 μg g−1, respectively. Sample analysis using sector field inductively coupled plasma-mass spectrometry (ICP-MS) was carried out to generate comparative data to that obtained using the chromatographic method.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:McGillicuddy, N (Ms Nicola McGillicuddy)
Author:Jones, P (Mr Phil Jones)
Author:Caldarola, D (Mr Dario Caldarola)
Author:Townsend, AT (Associate Professor Ashley Townsend)
Author:Mitev, DP (Dr Dimitar Mitev)
Author:Nesterenko, PN (Professor Pavel Nesterenko)
Author:Paull, B (Professor Brett Paull)
ID Code:84717
Year Published:2013
Web of Science® Times Cited:4
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-05-29
Last Modified:2014-06-23
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