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Staphylococcus aureus uses a novel multidomain receptor to break apart human hemoglobin and steal its heme

Citation

Spirig, T and Malmirchegini, GR and Zhang, J and Robson, SA and Sjodt, M and Liu, M and Kumar, KK and Dickson, CF and Gell, DA and Lei, B and Loo, JA and Clubb, RT, Staphylococcus aureus uses a novel multidomain receptor to break apart human hemoglobin and steal its heme, Journal of Biological Chemistry, 288, (2) pp. 1065-1078. ISSN 0021-9258 (2013) [Refereed Article]


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Copyright Statement

Copyright 2013 The American Society for Biochemistry and Molecular Biology, Inc

DOI: doi:10.1074/jbc.M112.419119

Abstract

Staphylococcus aureus is a leading cause of life-threatening infections in the United States. It requires iron to grow, which must be actively procured from its host to successfully mount an infection. Heme-iron within hemoglobin (Hb) is the most abundant source of iron in the human body and is captured by S. aureus using two closely related receptors, IsdH and IsdB. Here we demonstrate that each receptor captures heme using two conserved near iron transporter (NEAT) domains that function synergistically. NMR studies of the 39-kDa conserved unit from IsdH (IsdHN2N3, Ala326-Asp660) reveals that it adopts an elongated dumbbell-shaped structure in which its NEAT domains are properly positioned by a helical linker domain, whose three-dimensional structure is determined here in detail. Electrospray ionization mass spectrometry and heme transfer measurements indicate that IsdHN2N3 extracts heme from Hb via an ordered process in which the receptor promotes heme release by inducing steric strain that dissociates the Hb tetramer. Other clinically significant Gram-positive pathogens capture Hb using receptors that contain multiple NEAT domains, suggesting that they use a conserved mechanism. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

Item Details

Item Type:Refereed Article
Keywords:hemoglobin, NEAT domain, iron uptake, heme uptake
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Structural Biology (incl. Macromolecular Modelling)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Dickson, CF (Miss Claire Dickson)
Author:Gell, DA (Dr David Gell)
ID Code:84535
Year Published:2013
Web of Science® Times Cited:25
Deposited By:Menzies Institute for Medical Research
Deposited On:2013-05-17
Last Modified:2014-04-17
Downloads:0

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