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Development and experimental application of an HPLC procedure for the determination of capsaicin and dihydrocapsaicin in serum samples from human subjects

Citation

Hartley, T and Stevens, B and Ahuja, KDK and Ball, MJ, Development and experimental application of an HPLC procedure for the determination of capsaicin and dihydrocapsaicin in serum samples from human subjects, Indian Journal of Clinical Biochemistry, 28, (4) pp. 329-335. ISSN 0970-1915 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Association of Clinical Biochemists of India

DOI: doi:10.1007/s12291-013-0297-0

Abstract

Total capsaicins are extracted from 2 mL aliquots of serum or plasma using methyl-isobutyl ketone, evaporation of the extract to dryness and reconstitution with 200 lL of acetonitrile. The HPLC mobile phase is 40:60 water:acetonitrile. The absorbance of the eluent is monitored at 205 nm. Standardisation uses a known mixture of pure capsaicin and dihydrocapsaicin. Accuracies are 98.9 and 100.6 % for capsaicin and dihydrocapsaicin respectively. Inter batch reproducibility for both is 15 %. The limits of detection are 2.6 and 3.8 ng/mL for capsaicin and dihydrocapsaicin respectively. Analyses of sera obtained previously from human subjects who had eaten chilli containing meals showed that in those that absorbed capsaicins (N = 30) then the median, mean and SD of their serum capsaicin were: 13.4, 18.9 and 16.3 ng/mL. The corresponding data for those sera (N = 13) that had measurable levels of dihydrocapsaicin were: 6.9, 7.5 and 3.6 ng/mL. This procedure is suitable for use in prospective studies of the metabolism of orally ingested chilli.

Item Details

Item Type:Refereed Article
Keywords:HPLC, Capsaicin, Dihydrocapsaicin, serum levels, post oral dose
Research Division:Medical and Health Sciences
Research Group:Medical Biochemistry and Metabolomics
Research Field:Medical Biochemistry and Metabolomics not elsewhere classified
Objective Division:Health
Objective Group:Public Health (excl. Specific Population Health)
Objective Field:Nutrition
Author:Hartley, T (Dr Thomas Hartley)
Author:Stevens, B (Dr Brian Stevens)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Ball, MJ (Professor Madeleine Ball)
ID Code:84532
Year Published:2013
Deposited By:Health Sciences A
Deposited On:2013-05-16
Last Modified:2014-04-23
Downloads:0

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