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Isolation of RAG-1 and IgM transcripts from the striped trumpeter (Latris Lineata), and their expression as markers for the development of the adaptive immune response

Citation

Covello, J and Bird, S and Morrison, RN and Bridle, AR and Battaglene, SC and Secombes, CJ and Nowak, BF, Isolation of RAG-1 and IgM transcripts from the striped trumpeter (Latris Lineata), and their expression as markers for the development of the adaptive immune response, Fish and Shellfish Immunology, 34, (3) pp. 778-788. ISSN 1050-4648 (2013) [Refereed Article]

Copyright Statement

Copyright 2013 Elsevier Ltd.

DOI: doi:10.1016/j.fsi.2012.12.015

Abstract

A partial sequence of the recombination activating gene-1 (RAG-1) and several full length sequences of the immunoglobulin M (IgM) heavy chain mRNA were obtained from the striped trumpeter (Latris lineata). The RAG-1 fragment consisted of 205 aa and fell within the core region of the open reading frame. The complete IgM heavy chain sequences translated into peptides ranging between 581 and 591 aa. Both genes showed good homology to other vertebrate sequences. The expression of the two genes was assessed throughout the early developmental stages of striped trumpeter larvae (5e100 dph) and used as markers to follow the ontogeny of the adaptive immune response. Using RT-PCR, RAG-1 mRNA expression was detectable at 5 dph and remained so until 80 dph, before becoming undetectable at 100 dph. IgM expression was also detectable at 5 dph, and remained so throughout. These patterns of expression may suggest that the striped trumpeter possess mature B cells with surface IgM at 100 dph. However, complete immunological competence is likely not reached until some time later. The early detection of IgM mRNA at 5 dph led to the investigation of its presence in oocytes. Both RAG-1 and IgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biological significance of such a phenomenon remains to be investigated.

Item Details

Item Type:Refereed Article
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - Aquaculture
Objective Field:Aquaculture Crustaceans (excl. Rock Lobster and Prawns)
Author:Covello, J (Ms Jennifer Covello)
Author:Morrison, RN (Dr Richard Morrison)
Author:Bridle, AR (Dr Andrew Bridle)
Author:Battaglene, SC (Associate Professor Stephen Battaglene)
Author:Nowak, BF (Professor Barbara Nowak)
ID Code:84310
Year Published:2013
Web of Science® Times Cited:2
Deposited By:NC Marine Conservation and Resource Sustainability
Deposited On:2013-05-06
Last Modified:2014-04-28
Downloads:0

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