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Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K+ loss via GORK channel
Citation
Jayakannan, M and Bose, J and Babourina, O and Rengel, Z and Shabala, S, Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K+ loss via GORK channel, Journal of Experimental Botany, 64, (8) pp. 2255-2268. ISSN 0022-0957 (2013) [Refereed Article]
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Copyright Statement
Copyright 2013 the authors.
Abstract
Despite numerous reports implicating salicylic acid (SA) in plant salinity responses, the specific ionic mechanisms of SA-mediated adaptation to salt stress remain elusive. To address this issue, a non-invasive microelectrode ion flux estimation technique was used to study kinetics of NaCl-induced net ion fluxes in Arabidopsis thaliana in response to various SA concentrations and incubation times. NaCl-induced K+ efflux and H+ influx from the mature root zone
were both significantly decreased in roots pretreated with 10–500 μM SA, with strongest effect being observed in the 10–50 μM SA range. Considering temporal dynamics (0–8-h SA pretreatment), the 1-h pretreatment was most effective
in enhancing K+ retention in the cytosol. The pharmacological, membrane potential, and shoot K+ and Na+ accumulation data were all consistent with the model in which the SA pretreatment enhanced activity of H+-ATPase, decreased
NaCl-induced membrane depolarization, and minimized NaCl-induced K+ leakage from the cell within the first hour of salt stress. In long-term treatments, SA increased shoot K+ and decreased shoot Na+ accumulation. The shortterm
NaCl-induced K+ efflux was smallest in the gork1-1 mutant, followed by the rbohD mutant, and was highest in the wild type. Most significantly, the SA pretreatment decreased the NaCl-induced K+ efflux from rbohD and the wild
type to the level of gork1-1, whereas no effect was observed in gork1-1. These data provide the first direct evidence that the SA pretreatment ameliorates salinity stress by counteracting NaCl-induced membrane depolarization and by
decreasing K+ efflux via GORK channels.
Item Details
Item Type: | Refereed Article |
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Keywords: | H+-ATPase, H+ flux, depolarization, K+ flux, membrane potential, outward-rectifying K+ channel, potassium homeostasis |
Research Division: | Biological Sciences |
Research Group: | Plant biology |
Research Field: | Plant physiology |
Objective Division: | Plant Production and Plant Primary Products |
Objective Group: | Environmentally sustainable plant production |
Objective Field: | Environmentally sustainable plant production not elsewhere classified |
UTAS Author: | Jayakannan, M (Mrs Maheswari Jayakannan) |
UTAS Author: | Bose, J (Dr Jayakumar Bose) |
UTAS Author: | Shabala, S (Professor Sergey Shabala) |
ID Code: | 84146 |
Year Published: | 2013 |
Web of Science® Times Cited: | 106 |
Deposited By: | Tasmanian Institute of Agriculture |
Deposited On: | 2013-04-22 |
Last Modified: | 2015-02-04 |
Downloads: | 472 View Download Statistics |
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