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Effect of in vivo exposure to allogeneic cells upon subsequent in vitro T cell responses and upon allograft rejection

Citation

Cooley, MA, Effect of in vivo exposure to allogeneic cells upon subsequent in vitro T cell responses and upon allograft rejection, Scandinavian Journal of Immunology, 7, (5) pp. 371-80. ISSN 0300-9475 (1978) [Refereed Article]

DOI: doi:10.1111/j.1365-3083.1978.tb00467.x

Abstract

The effect of intravenous injection of 10(6) BALB/c spleen cells into C57B1/6J recipients was assayed by both mixed lymphocyte culture (MLC) of recipient lymphocytes, and by grafting donor (BALB/c) thyroids into recipient mice. It was observed that a single intravenous injection produced depression of proliferative and cytotoxic T cell responses in MLC of spleen, lymph node and peripheral blood lymphocytes of the recipients. This effect was specific for the sensitizing genotype (MLC of recipient and third-party CBA/H lymphocytes was unaffected), and persisted for several days after sensitization. The pattern of this diminished response suggested that the effect was due to a combination of recruitment of reactive lymphocytes from peripheral lymphoid populations, and the generation of alloantigen (H-2?)-specific suppressor T cells in the spleen. In contrast to these findings in vitro, a similar sensitization led only to accelerated rejection of thyroid allografts in vivo.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
Author:Cooley, MA (Associate Professor Margaret Cooley)
ID Code:83902
Year Published:1978
Web of Science® Times Cited:8
Deposited By:Medicine (Discipline)
Deposited On:2013-03-26
Last Modified:2013-03-26
Downloads:0

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