We examined blood mononuclear cells from six recipients of HLA-identical sibling marrow transplants for their ability to express messenger (m) RNA for granulocyte-macrophage colony stimulating factor (GM-CSF). Cells were cultured for 48 hours with the stimulus of ionomycin and phorbol myristate acetate. mRNA specific for GM-CSF was detectable in stimulated cell cultures from all six patients. In two patients at days 31 and 40 post transplant respectively, mRNA for GM-CSF was just detectable; in a third at day 38 post transplant it was readily detectable, and in the three others at days 42, 165 and 185 respectively, it was easily detectable. These results indicate that maintenance of haemopoiesis post transplant may, at least in part, be due to the elaboration of GM-CSF by blood mononuclear cells.

Item Type:	Refereed Article
Research Division:	Medical and Health Sciences
Research Group:	Immunology
Research Field:	Cellular Immunology
Objective Division:	Health
Objective Group:	Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:	Infectious Diseases
Author:	Cooley, MA (Associate Professor Margaret Cooley)
ID Code:	83881
Year Published:	1988
Deposited By:	Medicine (Discipline)
Deposited On:	2013-03-26
Last Modified:	2013-03-26
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