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Expression of interleukin 5 by the CD4+CD45R0+ subset of human T cells


Sewell, WA and Valentine, JE and Cooley, MA, Expression of interleukin 5 by the CD4+CD45R0+ subset of human T cells, Growth Factors, 6, (4) pp. 295 - 302. ISSN 0897-7194 (1992) [Refereed Article]

DOI: doi:10.3109/08977199209021541


The expression of IL5 by CD4+CD45RA+, CD4+CD45R0+ and CD3+CD8+ subsets of human peripheral blood mononuclear cells was assessed. Interleukin 5 expression was detected by RNA extraction, reverse transcription and polymerase chain reaction. Populations of highly purified cells were obtained by a protocol of sequential plastic adherence, magnetic bead separation and flow cytometric cell sorting. IL5 was clearly expressed in the CD4+CD45R0+ subset from 3 to 48 hr after activation. The CD4+CD45RA+ and CD3+CD8+ subsets expressed very much less IL5. By contrast, IL2 expression was readily detected in all sorted populations. Thus, in activated CD4+ cells, IL5 was predominantly expressed in the CD4+CD45R0+ subset, a pattern of expression corresponding to that reported for a number of other cytokines, and differing from that of IL2.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Cooley, MA (Associate Professor Margaret Cooley)
ID Code:83874
Year Published:1992
Web of Science® Times Cited:18
Deposited By:Medicine
Deposited On:2013-03-26
Last Modified:2013-03-26

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