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IL-16 regulation of humanmast cells/basophils and their susceptibility to HIV-1


Qi, JC and Stevens, RL and Wadley, R and Collins, A and Cooley, MA and Naif, HM and Nasr, N and Cunningham, A and Katsoulotos, G and Wanigasek, Y and Roufogalis, B and Krilis, SA, IL-16 regulation of humanmast cells/basophils and their susceptibility to HIV-1, Journal of Immunology, 168, (8) pp. 4127 - 4134. ISSN 0022-1767 (2002) [Refereed Article]

DOI: doi:10.4049/jimmunol.168.8.4127


AIDS patients often contain HIV-1-infected mast cells (MCs)/basophils in their peripheral blood, and in vivo-differentiated MCs/basophils have been isolated from the blood of asthma patients that are HIV-1 susceptible ex vivo due to their surface expression of CD4 and varied chemokine receptors. Because IL-16 is a ligand for CD4 and/or an undefined CD4-associated protein, the ability of this multifunctional cytokine to regulate the development of human MCs/basophils from nongranulated progenitors residing in cord or peripheral blood was evaluated. After 3 wk of culture in the presence of c-kit ligand, IL-16 induced the progenitors residing in the blood of normal individuals to increase their expression of chymase and tryptase about 20-fold. As assessed immunohistochemically, >80% of these tryptase(+) and/or chymase(+) cells expressed CD4. The resulting cells responded to IL-16 in an in vitro chemotaxis assay, and this biologic response could be blocked by anti-IL-16 and anti-CD4 Abs as well as by a competitive peptide inhibitor corresponding to a sequence in the C-terminal domain of IL-16. The additional finding that IL-16 induces calcium mobilization in the HMC-1 cell line indicates that IL-16 acts directly on MCs and their committed progenitors. IL-16-treated MCs/basophils also are less susceptible to infection by an M/R5-tropic strain of HIV-1. Thus, IL-16 regulates MCs/basophils at a number of levels, including their vulnerability to retroviral infection.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Cooley, MA (Associate Professor Margaret Cooley)
ID Code:83738
Year Published:2002
Web of Science® Times Cited:25
Deposited By:Medicine
Deposited On:2013-03-21
Last Modified:2013-03-21

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