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T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation


Eri, RD and McGuckin, MA and Wadley, R, T cell transfer model of colitis: a great tool to assess the contribution of T cells in chronic intestinal inflammation, Leucocytes: Methods and Protocols, Methods in Molecular Biology, Humana Press, Robert B. Ashman (ed), United States, pp. 261-275. ISBN 978-1-61779-526-8 (2012) [Research Book Chapter]

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Copyright 2012 Springer Science+Business Media, LLC

DOI: doi:10.1007/978-1-61779-527-5_19


Inflammatory bowel diseases (IBD) consist of Crohn's disease (CD) and ulcerative colitis (UC) affecting about 0.1% of the western population. These two chronic gut diseases affect youth at their prime of life causing diarrhoea, intestinal bleeding, and severe gut discomfort. Mouse models of colitis have been major tools in understanding the pathogenesis of IBD. A number of mouse models are available to assess the contribution of T cells in the pathogenesis of CD and UC. Among these, the T cell transfer model of colitis is the most widely used model to dissect the initiation, induction, and regulation of immunopathology in chronic colitis mediated by T cells. The methodology below describes the classification of various animal models and explains the T cell transfer model in detail, including flow cytometry-based isolation of na´ve T cells that are used in the transfer, immunological concepts, detailed immune-pathological assessment, shortcomings of the model, and the latest improvements to this colitis model. A special focus is paid to the utilisation of the T cell transfer model in delineating the immunopathology in a primary epithelial defect model of colitis, namely Winnie.

Item Details

Item Type:Research Book Chapter
Keywords:T cells, colitis, IBD
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genetic immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Eri, RD (Associate Professor Raj Eri)
ID Code:83575
Year Published:2012
Funding Support:National Health and Medical Research Council (604304)
Deposited By:Health Sciences A
Deposited On:2013-03-19
Last Modified:2017-10-16

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