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Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems


Newman, I and Chen, S-L and Porterfield, M and Sun, J, Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations, and Systems, Plant Salt Tolerance: Methods and Protocols, Humana Press, Shabala and TA Cuin (ed), New York, pp. 101-117. ISBN 978-1-61779-985-3 (2012) [Research Book Chapter]

Copyright Statement

Copyright 2012 Springer Science+Business Media, LLC

DOI: doi:10.1007/978-1-61779-986-0_6


Knowledge of the fluxes of ions and neutral molecules across the outer membrane or boundary of living tissues and cells is an important strand of applied molecular biology. Such fluxes can be measured non-invasively with good resolution in time and space. Two systems (MIFE™ and SIET) have been developed and have become widely used to implement this technique, and they are commercially available. This Chapter is the first comparative description of these two systems. It gives the context, the basic underlying theory, practical limitations inherent in the technique, theoretical developments, guidance on the practicalities of the technique, and the functionality of the two systems. Although the technique is strongly relevant to plant salt tolerance and other plant stresses (drought, temperature, pollutants, waterlogging), it also has rich relevance throughout biomedical studies and the molecular genetics of transport proteins.

Item Details

Item Type:Research Book Chapter
Keywords:ISE, MIFE, SIET, salinity ion flux electrophysiology, membrane transport, ion selective microelectrodes, microsensors, self-referencing
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Newman, I (Dr Ian Newman)
ID Code:83533
Year Published:2012
Deposited By:Mathematics and Physics
Deposited On:2013-03-18
Last Modified:2017-10-20

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