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Field enhanced bacterial sample stacking in isotachophoresis using wide-bore capillaries

Citation

Oukacine, F and Quirino, JP and Destoumieux-Garzon, D and Cottet, H, Field enhanced bacterial sample stacking in isotachophoresis using wide-bore capillaries, The Journal of Chromatography A, 1268 pp. 180-184. ISSN 0021-9673 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier B.V.

DOI: doi:10.1016/j.chroma.2012.10.026

Abstract

The isotachophoretic analysis of different bacterial strains was studied using capillaries with different internal diameters from 50 to 250レm. Several injection modes were investigated and compared in order to improve the limit of detection of bacteria by capillary isotachophoresis. A system suitability test obtained from the separation voltage was developed to ensure reliable results. As expected, the use of wider bore capillaries improved the analytical sensitivity of the isotachophoretic method when compared to the 50レm capillary. With the optimized conditions, the isotachophoretic method presented in this work allows the quantification of Erwinia carotovora (Gram negative bacteria) with a limit of detection as low as ´3000 cells mL−1. The proposed methodology does not require any additive in the electrolyte such a fluorescent or chromophoric dye to reach these limits of detection.

Item Details

Item Type:Refereed Article
Keywords:capillary zone electrophoresis, isotachophoresis, bacteria, electrokinetic injection, field-enhanced sample injection
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:83366
Year Published:2012
Web of Science® Times Cited:6
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-03-12
Last Modified:2017-10-29
Downloads:0

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