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Simultaneous electrokinetic and hydrodynamic injection with on-line sample concentration via micelle to solvent stacking in micellar electrokinetic chromatography

Citation

Quirino, JP and Aranas, AT, Simultaneous electrokinetic and hydrodynamic injection with on-line sample concentration via micelle to solvent stacking in micellar electrokinetic chromatography, Analytica Chimica Acta: International Journal Devoted to All Branches of Analytical Chemistry, 733 pp. 84-89. ISSN 0003-2670 (2012) [Refereed Article]

Copyright Statement

Crown Copyright 2012

DOI: doi:10.1016/j.aca.2012.04.032

Abstract

Simultaneous electrokinetic and hydrodynamic injection (SEHI) of organic cations (tricyclic antidepressant and beta blocker drugs) with on-line sample concentration using micelle to solvent stacking (MSS) was studied in micellar electrokinetic chromatography. Compared to conventional injection, >300-fold improvements in signals were obtained by hydrodynamic injection. However, with SEHI the amount of sample ions introduced into the capillary was increased which afforded a higher gain of up to 4000-fold without compromise to separation efficiency. The electrokinetic injection at negative polarity (anode at the detector end) introduced the micelle bound analytes. The hydrodynamic injection also maintained the MSS boundary inside the capillary. The stability of the MSS boundary affected SEHI where mild conditions that were low voltage as well as pressure injection were desired. The limits of detection were in the range from 0.64.2 ng mL−1. A strategy for optimization was described and the method was applied to the ng mL−1 analysis of spiked wastewater after simple dilution and centrifugation.

Item Details

Item Type:Refereed Article
Keywords:on-line sample concentration, micelle to solvent stacking, capillary electrophoresis, cations, drugs, simultaneous electrokinetic and hydrodynamic injection
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Quirino, JP (Associate Professor Lito Quirino)
Author:Aranas, AT (Miss T Aranas)
ID Code:83358
Year Published:2012
Web of Science® Times Cited:13
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-03-12
Last Modified:2017-10-31
Downloads:0

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