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Synergistic effect of field enhanced sample injection on micelle to solvent stacking in capillary electrophoresis


Rabanes, HR and Aranas, AT and Benbow, NL and Quirino, JP, Synergistic effect of field enhanced sample injection on micelle to solvent stacking in capillary electrophoresis, The Journal of Chromatography A, 1267 pp. 74-79. ISSN 0021-9673 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Published by Elsevier B.V.

DOI: doi:10.1016/j.chroma.2012.08.073


Injection of a sodium dodecyl sulfate micellar plug prior to the sample solution induced transient micellar phase extraction of cationic drugs in capillary electrophoresis. Micelle to solvent stacking mechanism was utilized by preparing the sample in aqueous organic solvent. Synergism was achieved with field enhanced sample injection that allowed larger sample loads. Micelle to solvent stacking occurred concurrently with the enhanced injection after the fast moving analytes electrophoretically migrated into the micellar plug. This was different from stacking combinations with field enhancement where the electric field strength difference was involved in analyte focusing. Using a sample diluent that had one-tenth the conductivity of the background solution, the strategy afforded thousands-fold improvements in peak height and LODs (S/N = 3) of as low as 1.1 ng/mL. The results were repeatable and linear. Adaptability to real sample analysis was evaluated using spiked urine sample after minimal sample clean-up.

Item Details

Item Type:Refereed Article
Keywords:antipsychotic drugs, capillary electrophoresis, field enhanced sample injection, organic solvent, micelle to solvent stacking, on-line sample concentration, transient micellar phase concentrator
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Rabanes, HR (Ms Heide Rabanes)
UTAS Author:Aranas, AT (Miss T Aranas)
UTAS Author:Benbow, NL (Miss Natalie Benbow)
UTAS Author:Quirino, JP (Associate Professor Lito Quirino)
ID Code:83356
Year Published:2012
Web of Science® Times Cited:26
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-03-12
Last Modified:2017-10-31

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