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Testosterone Determination Using Rapid Heterogeneous Competitive-Binding for Enzyme-Linked Immunosorbent Assay in Flow Injection

Citation

Dimitrakopoulos, LT and Dimitrakopoulos, T and Davy, RM and Iles, PJ, Testosterone Determination Using Rapid Heterogeneous Competitive-Binding for Enzyme-Linked Immunosorbent Assay in Flow Injection, Analytical Letters, 29, (12) pp. 2125-2139. ISSN 0003-2719 (1996) [Refereed Article]

DOI: doi:10.1080/00032719608002236

Abstract

The application of a rapid competitive enzyme-linked immunosorbent assay (ELISA) for the measurement of testosterone in flow injection (FI) is presented. The ELISA protocol for the determination of testosterone was performed in 60 min compared to the standard protocol time of 150 min. This was achieved by reducing the antibody incubation time. The ELISA was used successfully to determine testosterone levels in clinical samples of both male and female subjects, and were in good agreement compared to a pathology laboratory which employed an immunoradiometric assay (IRMA).

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Instrumental Methods (excl. Immunological and Bioassay Methods)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Dimitrakopoulos, LT (Mrs Lucy Tina Dimitrakopoulos)
Author:Dimitrakopoulos, T (Mr Telis Dimitrakopoulos)
ID Code:8322
Year Published:1996
Web of Science® Times Cited:3
Deposited By:Applied Science
Deposited On:1996-08-01
Last Modified:2011-08-19
Downloads:0

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