eCite Digital Repository

Potentiometric detection using a metallic copper electrode in reversed-phase and ion-exclusion chromatography with eluents containing ion-interaction reagents

Citation

Glod, KB and Alexander, PW and Haddad, PR and Chen, ZL, Potentiometric detection using a metallic copper electrode in reversed-phase and ion-exclusion chromatography with eluents containing ion-interaction reagents, Journal of Chromatography A, 699, (1-2) pp. 31-37. ISSN 0021-9673 (1995) [Refereed Article]

DOI: doi:10.1016/0021-9673(95)00046-P

Abstract

The response characteristics of a metallic copper wire electrode used as a sensor electrode in a potentiometric detector after ion-interaction separations are determined. Volatile carboxylic acids have been selected as model compounds to test the sensor electrode. It is found that concentrations of an ion-interaction reagent (tetraethylammonium perchlorate) as small as 0.01 mM in an unbuffered mobile phase are optimal for the potentiometric detection and separation in two chromatographic systems, namely reversed-phase and ion-exclusion. The ion-interaction reagent gives improved solute peak shape and results in retention times becoming independent of solute concentration, without any adverse effect on detection sensitivity. An increase in the mobile phase concentration of the reagent results in increased solute retention in the ion-exclusion chromatography mode and variation of this concentration is a new parameter that can be used to control solute retention. Typical detection limits are 250, 510 and 360 pmol for acetic, propionic and butyric acids, respectively.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Instrumental Methods (excl. Immunological and Bioassay Methods)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Alexander, PW (Professor Peter Alexander)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:8321
Year Published:1995
Web of Science® Times Cited:17
Deposited By:Applied Science
Deposited On:1995-08-01
Last Modified:2011-08-25
Downloads:0

Repository Staff Only: item control page