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Reversed phase high performance liquid chromatographic determination of dissolved aluminium in open ocean seawater


Remenyi, T and Nesterenko, P and Bowie, A and Butler, E and Haddad, P, Reversed phase high performance liquid chromatographic determination of dissolved aluminium in open ocean seawater, Limnology and Oceanography: Methods, 10 pp. 832-839. ISSN 1541-5856 (2012) [Refereed Article]

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Copyright Statement

Copyright 2012 American Society of Limnology and Oceanography, Inc.

DOI: doi:10.4319/lom.2012.10.832


Popular shipboard techniques to determine dissolved aluminium in open ocean seawater have two major disadvantages: relative to HPLC techniques, they require large sample volumes; and, in practice they are only semi-automated. To address these two issues, the application of a reversed-phase high performance liquid chromatographic (RP-HPLC) method for the determination of the aluminium in open ocean seawater was developed. With this method, dissolved aluminium in the sample was complexed with lumogallion at pH 5.5 by the addition of a reagent, producing the fluorescent aluminium-lumogallion complex. This complex was concentrated onto a Chromolith RP-18e Guard (10 x 4.6 mm) column and eluted with 95% methanol into a fluorescence detector (λex=505 nm, λem=574 nm). Sample pre-treatment required 10 h (>100 samples can be prepared simultaneously) and sample analysis required 375 μL of sample per vial analysed, with 10 μL and 2.7 min per injection.Total required sample volume (including rinses) was 1.5 mL. Limit of detection was 0.130.05 nM, with precision of 2.7% at 1 nM on SAFe reference samples. Agreement with SAFe reference samples was within 4.64.6% (n=13). The technique is robust, inherently automated and could easily be applied shipboard for open ocean analyses. It provides improvements in sample efficiency, operational robustness and automation relative to popular shipboard techniques, especially when applied to samples with dissolved aluminium concentrations that approach the limit of detection. This project was a contribution to the GEOTRACES program.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Remenyi, T (Dr Tom Remenyi)
UTAS Author:Nesterenko, P (Professor Pavel Nesterenko)
UTAS Author:Bowie, A (Professor Andrew Bowie)
UTAS Author:Butler, E (Dr Edward Butler)
UTAS Author:Haddad, P (Professor Paul Haddad)
ID Code:82809
Year Published:2012
Web of Science® Times Cited:2
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-02-19
Last Modified:2016-10-26
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