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Preliminary evidence for the microbial loop in Antarctic sea ice using microcosm simulations

Citation

Martin, A and McMinn, A and Davy, SK and Anderson, MJ and Miller, HC and Hall, JA and Ryan, KG, Preliminary evidence for the microbial loop in Antarctic sea ice using microcosm simulations, Antarctic Science, 24, (6) pp. 547-553. ISSN 0954-1020 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Antarctic Science

DOI: doi:10.1017/S0954102012000491

Abstract

Sea ice microalgae actively contribute to the pool of dissolved organic matter (DOM) available for bacterial metabolism, but this link has historically relied on bulk correlations between chlorophyll a (a surrogate for algal biomass) and bacterial abundance. We incubated microbes from both the bottom (congelation layer) and surface brine region of Antarctic fast ice for nine days. Algal-derived DOM was manipulated by varying the duration of irradiance, restricting photosynthesis with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) or incubating in the dark. The bacterial response to changes in DOM availability was examined by performing cell counts, quantifying bacterial metabolic activity and examining community composition with denaturing gradient gel electrophoresis. The percentage of metabolically active bacteria was relatively low in the surface brine microcosm (10–20% of the bacterial community), the treatment with DCMU indirectly restricted bacterial growth and there was some evidence for changes in community structure. Metabolic activity was higher (35–69%) in the bottom ice microcosm, and while there was no variation in community structure, bacterial growth was restricted in the treatment with DCMU compared to the light/dark treatment. These results are considered preliminary, but provide a useful illustration of sea ice microbial dynamics beyond the use of ‘snapshot’ biomass correlations.

Item Details

Item Type:Refereed Article
Keywords:Antarctic, microbial loop, bacteria, DGGE, microalgae
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and Estuarine Ecology (incl. Marine Ichthyology)
Objective Division:Environment
Objective Group:Flora, Fauna and Biodiversity
Objective Field:Antarctic and Sub-Antarctic Flora, Fauna and Biodiversity
Author:Martin, A (Dr Andrew Martin)
Author:McMinn, A (Professor Andrew McMinn)
ID Code:82711
Year Published:2012
Web of Science® Times Cited:2
Deposited By:IMAS Research and Education Centre
Deposited On:2013-02-14
Last Modified:2017-10-31
Downloads:1 View Download Statistics

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