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Validation of diagnostic assays to screen broodstock for Flavobacterium psychrophilum infections
Citation
Long, A and Polinski, MP and Call, DR and Cain, KD, Validation of diagnostic assays to screen broodstock for Flavobacterium psychrophilum infections, Journal of Fish Diseases, 35, (6) pp. 407-419. ISSN 1365-2761 (2012) [Refereed Article]
Copyright Statement
Copyright 2012 Blackwell Publishing Ltd
DOI: doi:10.1111/j.1365-2761.2012.01357.x
Abstract
It is hypothesized that the frequency of bacterial
coldwater disease outbreaks can be reduced through
the detection of the aetiologic agent, Flavobacterium
psychrophilum, in broodstock followed by culling of
eggs from heavily infected broodstock. Before a
culling programme can be instituted, however, it is
necessary to determine the sensitivity and specificity
of existing assays for the detection of F. psychrophilum.
In this study, tissue and ovarian fluid
samples were collected from 224 fish at five
hatcheries and screened using an enzyme-linked
immunosorbent assay (ELISA), a membrane-filtration
fluorescent antibody test (MF-FAT), bacteriological
culture and nested PCR. Latent class
analysis was used to estimate sensitivity and specificity
of kidney culture, kidney ELISA, nested PCR
and MF-FAT. Analytical sensitivity of the ELISA
varied but was greatest when bacteria were cultured
under iron-limiting conditions. Diagnostic sensitivity
estimates ranged from 0.02 (kidney culture)
to 0.97 (kidney ELISA). Specificity estimates ranged
from 0.02 (MF-FAT) to 0.98 (kidney ELISA).
In a separate challenge experiment, the ELISA
confirmed the presence of F. psychrophilum in subclinically
infected fish. Results from this study
demonstrate that the ELISA is an appropriate tool
to screen broodstock and provides an indication of
infection severity, which is crucial for implementation
of a screening/culling programme.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | coldwater disease, disease prevalence, ELISA, Flavobacterium psychrophilum, membrane-filtration FAT |
| Research Division: | Biological Sciences |
| Research Group: | Microbiology |
| Research Field: | Infectious Agents |
| Objective Division: | Animal Production and Animal Primary Products |
| Objective Group: | Fisheries - Aquaculture |
| Objective Field: | Aquaculture Crustaceans (excl. Rock Lobster and Prawns) |
| UTAS Author: | Polinski, MP (Mr Mark Polinski) |
| UTAS Author: | Cain, KD (Associate Professor ken Cain) |
| ID Code: | 82538 |
| Year Published: | 2012 |
| Web of Science® Times Cited: | 6 |
| Deposited By: | NC Marine Conservation and Resource Sustainability |
| Deposited On: | 2013-02-06 |
| Last Modified: | 2013-05-22 |
| Downloads: | 0 |
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