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Anti-β2GP1 antibodies have variable effects on platelet aggregation

Citation

Betts, NA and Ahuja, KDK and Adams, MJ, Anti-β2GP1 antibodies have variable effects on platelet aggregation, Pathology, 45, (2) pp. 155-161. ISSN 0031-3025 (2013) [Refereed Article]

Copyright Statement

2013 Royal College of Pathologists of Australasia

DOI: doi:10.1097/PAT.0b013e32835cc277

Abstract

Aim: To investigate the effects of affinity-purified rabbit antib2GP1, and anti-b2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate(ADP)-induced aggregation. Methods: Whole blood was collected and processed to obtain platelet poor plasma PPP) from normal controls(n15) and SLE patients (n15). Using PPP, anti-b2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-b2GP1 was purified from two SLE patients using purified b2GP1 coupled to a HiTrap NHS-activated HP column. Results: The effect of rabbit and human derived antib2GP1(0100mg/mL), on ADP(2.5, 5mM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-b2GP1 significantly inhibited all parameters of 5mM ADP-induced platelet aggregation; %Max ( p0.028), %AUC ( p0.014) and slope ( p<0.001). In contrast, anti-b2GP1 purified from SLE patients significantly enhanced the %Max ( p0.031)and %AUC ( p0.007) in a concentration dependent manner,but inhibited the slope ( p<0.05) of 5mM ADP-induced platelet aggregation. Conclusion: Our data suggest anti-b2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-b2GP1 may be due to the heterogeneous nature of anti-b2GP1,varying avidity or different antibody binding specificities between species.

Item Details

Item Type:Refereed Article
Keywords:Platelets, antiphospholipid antibodies
Research Division:Medical and Health Sciences
Research Group:Cardiorespiratory Medicine and Haematology
Research Field:Haematology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Blood Disorders
Author:Betts, NA (Mrs Natasha Betts)
Author:Ahuja, KDK (Dr Kiran Ahuja)
Author:Adams, MJ (Dr Murray Adams)
ID Code:82514
Year Published:2013
Web of Science® Times Cited:3
Deposited By:Health Sciences A
Deposited On:2013-02-06
Last Modified:2017-11-06
Downloads:0

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