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Anti-β2GP1 antibodies have variable effects on platelet aggregation
Citation
Betts, NA and Ahuja, KDK and Adams, MJ, Anti-β2GP1 antibodies have variable effects on platelet aggregation, Pathology, 45, (2) pp. 155-161. ISSN 0031-3025 (2013) [Refereed Article]
Copyright Statement
2013 Royal College of Pathologists of Australasia
DOI: doi:10.1097/PAT.0b013e32835cc277
Abstract
Aim: To investigate the effects of affinity-purified rabbit antib2GP1, and anti-b2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate(ADP)-induced aggregation.
Methods: Whole blood was collected and processed to obtain platelet poor plasma PPP) from normal controls(n¼15) and SLE patients (n¼15). Using PPP, anti-b2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-b2GP1 was purified from two SLE patients using purified b2GP1 coupled to a HiTrap NHS-activated HP column.
Results: The effect of rabbit and human derived antib2GP1(0–100mg/mL), on ADP(2.5, 5mM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-b2GP1 significantly inhibited all parameters of 5mM ADP-induced platelet aggregation; %Max ( p¼0.028), %AUC ( p¼0.014) and slope ( p<0.001). In contrast, anti-b2GP1 purified from SLE patients significantly enhanced the %Max ( p¼0.031)and %AUC ( p¼0.007) in a concentration dependent manner,but inhibited the slope ( p<0.05) of 5mM ADP-induced platelet aggregation.
Conclusion: Our data suggest anti-b2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-b2GP1 may be due to the heterogeneous nature of anti-b2GP1,varying avidity or different antibody binding specificities between species.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Platelets, antiphospholipid antibodies |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Cardiovascular medicine and haematology |
| Research Field: | Haematology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Diagnosis of human diseases and conditions |
| UTAS Author: | Betts, NA (Mrs Natasha Betts) |
| UTAS Author: | Ahuja, KDK (Dr Kiran Ahuja) |
| UTAS Author: | Adams, MJ (Dr Murray Adams) |
| ID Code: | 82514 |
| Year Published: | 2013 |
| Web of Science® Times Cited: | 6 |
| Deposited By: | Health Sciences A |
| Deposited On: | 2013-02-06 |
| Last Modified: | 2017-11-06 |
| Downloads: | 0 |
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