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The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors

Citation

Yamaguchi, Y and Kaiya, H and Konno, N and Iwata, E and Miyazato, M and Uchiyama, M and Bell, JD and Toop, T and Donald, JA and Brenner, S and Venkatesh, B and Hyodo, S, The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors, General and Comparative Endocrinology, 178, (3) pp. 519-528. ISSN 0016-6480 (2012) [Refereed Article]

DOI: doi:10.1016/j.ygcen.2012.07.008

Abstract

The neurohypophysial peptides of the vasopressin (VP) and oxytocin (OT) families regulate salt and water homeostasis and reproduction through distinct G protein-coupled receptors. The current thinking is that there are four neurohypophysial hormone receptors (V1aR, V1bR, V2R, and OTR) in vertebrates, and their evolutionary history is still debated. We report the identification of a fifth neurohypophysial hormone receptor (V2bR) from the holocephalan elephant fish. This receptor is similar to conventional V2R (V2aR) in sequence, but induced Ca2+ signaling in response to vasotocin (VT), the non-mammalian VP ortholog; such signaling is typical of V1-type receptors. In addition, V1aR, V1bR and OTR were also isolated from the elephant fish. Further screening revealed that orthologous V2bRs are widely distributed throughout the jawed vertebrates, and that the V2bR family is subdivided into two subfamilies: the fish specific type-1, and a type-2 that is characteristically found in tetrapods. Analysis suggested that the mammalian V2bR may have lost its function. Based on molecular phylogenetic, synteny and functional analyses, we propose a new evolutionary history for the neurohypophysial hormone receptors in vertebrates as follows: the first duplication generated V1aR/V1bR/OTR and V2aR/V2bR lineages; after divergence from the V2bR lineage, the V2aRs evolved to use cAMP as a second messenger, while the V2bRs retained the original Ca2+ signaling system. Future studies on the role of V2bR in the brain, heart, kidney and reproductive organs, in which it is highly expressed, will open a new research field in VP/VT physiology and evolution. © 2012 Elsevier Inc..

Item Details

Item Type:Refereed Article
Keywords:Cartilaginous fish; G protein-coupled receptor; Molecular evolution; Neurohypophysial hormone; Vasotocin; argiprestocin; calcium ion; cyclic AMP; neurohypophysis hormone; oxytocin; oxytocin receptor; unclassified drug; vasopressin; animal tissue
Research Division:Agricultural and Veterinary Sciences
Research Group:Fisheries Sciences
Research Field:Fish Physiology and Genetics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
Author:Bell, JD (Dr Justin Bell)
ID Code:82452
Year Published:2012
Web of Science® Times Cited:23
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2013-02-04
Last Modified:2017-11-07
Downloads:0

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