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Phosphoenolpyruvate metabolism in Teladorsagia circumcincta: a critical junction between aerobic and anaerobic metabolism
Citation
Simcock, DC and Walker, LR and Pedley, KC and Simpson, HV and Brown, S, Phosphoenolpyruvate metabolism in Teladorsagia circumcincta: a critical junction between aerobic and anaerobic metabolism, Experimental Parasitology, 132, (2) pp. 267-273. ISSN 0014-4894 (2012) [Refereed Article]
Copyright Statement
Copyright 2012 Elsevier Inc.
DOI: doi:10.1016/j.exppara.2012.08.001
Abstract
Nematodes which have adapted to an anaerobic lifestyle in their adult stages oxidise phosphoenolpyruvate (PEP) to oxaloacetate rather than pyruvate as the final product of glycolysis. This adaptation involves selective expression of the enzyme phosphoenolpyruvate carboxykinase (PEPCK), instead of pyruvate kinase (PK). However, such adaptation is not absolute in aerobic nematode species. We have examined the activity and kinetics of PEPCK and PK in larvae (L3) and adults of Teladorsagia circumcincta, a parasite known to exhibit oxygen uptake. Results revealed that PK and PEPCK activity existed in both L3s and adults. The enzymes had differing affinity for nucleotide diphosphates: while both can utilise GDP, only PK utilised ADP and only PEPCK utilised IDP. In both life cycle stages, enzymes showed similar affinity for PEP. PK activity was predominant in both stages, although activity of this enzyme was lower in adults. When combined, both the activity levels and the enzyme kinetics showed that pyruvate production is probably favoured in both L3 and adult stages of T. circumcincta and suggest that metabolism of PEP to oxaloacetate is a minor metabolic pathway in this species.
Item Details
Item Type: | Refereed Article |
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Keywords: | phosphoenolpyruvate carboxykinase, pyruvate kinase, nematode, Teladorsagia circumcincta, energy metabolism |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Enzymes |
Objective Division: | Animal Production and Animal Primary Products |
Objective Group: | Livestock raising |
Objective Field: | Sheep for meat |
UTAS Author: | Brown, S (Dr Simon Brown) |
ID Code: | 82444 |
Year Published: | 2012 |
Web of Science® Times Cited: | 2 |
Deposited By: | Health Sciences A |
Deposited On: | 2013-02-04 |
Last Modified: | 2015-02-02 |
Downloads: | 0 |
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