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The kinetics and regulation of phosphofructokinase from Teladorsagia circumcincta

Citation

Walker, LR and Simcock, DC and Pedley, KC and Simpson, HV and Brown, S, The kinetics and regulation of phosphofructokinase from Teladorsagia circumcincta, Experimental Parasitology, 130, (4) pp. 348-353. ISSN 0014-4894 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 2012 Elsevier Inc.

DOI: doi:10.1016/j.exppara.2012.02.011

Abstract

Phosphofructokinase (PFK-1) activity was examined in L 3 and adult Teladorsagia circumcincta, both of which exhibit oxygen consumption. Although activities were higher in the adult stage, the kinetic properties of the enzyme were similar in both life cycle stages. T. circumcincta PFK-1 was subject to allosteric inhibition by high ATP concentration, which increased both the Hill coefficient (from 1.4±0.2 to 1.7±0.2 in L 3s and 2.0±0.3 to 2.4±0.4 in adults) and the K 1/2 for fructose 6 phosphate (from 0.35±0.02 to 0.75±0.05mM in L 3s and 0.40±0.03 to 0.65±0.05mM in adults). The inhibitory effects of high ATP concentration could be reversed by fructose 2,6 bisphosphate and AMP, but glucose 1,6 bisphosphate had no effect on activity. Similarly, phosphoenolpyruvate had no effect on activity, while citrate, isocitrate and malate exerted mild inhibitory effects, but only at concentrations exceeding 2mM. The observed kinetic properties for T. circumcincta PFK-1 were very similar to those reported for purified Ascaris suum PFK-1, though slight differences in sensitivity to ATP concentration suggests there may be subtle variations at the active site. These results are consistent with the conservation of properties of PFK-1 amongst nematode species, despite between species variation in the ability to utilise oxygen. © 2012 Elsevier Inc.

Item Details

Item Type:Refereed Article
Keywords:Teladorsagia circumcincta; Nematode; Phosphofructokinase
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Enzymes
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock Raising
Objective Field:Sheep - Meat
Author:Brown, S (Dr Simon Brown)
ID Code:82443
Year Published:2012
Web of Science® Times Cited:3
Deposited By:Health Sciences A
Deposited On:2013-02-04
Last Modified:2015-02-02
Downloads:0

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