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Determination of pharmaceutically related compounds by suppressed ion chromatography: IV. Interfacing ion chromatography with universal detectors

Citation

Karu, N and Hutchinson, JP and Dicinoski, GW and Hanna-Brown, M and Srinivasan, K and Pohl, CA and Haddad, PR, Determination of pharmaceutically related compounds by suppressed ion chromatography: IV. Interfacing ion chromatography with universal detectors, The Journal of Chromatography A, 1253 pp. 44-51. ISSN 0021-9673 (2012) [Refereed Article]

Copyright Statement

Crown Copyright 2012

DOI: doi:10.1016/j.chroma.2012.06.101

Abstract

This work forms the final part of a study investigating gradient elution ion-exchange chromatography of pharmaceutically relevant compounds, aiming at achieving complementary selectivity to reversed-phase HPLC. In this study the coupling of three universal detectors (electro-spray ionisation mass spectrometer (ESI-MS); corona charged aerosol detector (CAD); and evaporative light scattering detector (ELSD)) to suppressed IC using complex elution profiles with potassium hydroxide eluents is demonstrated. The non-volatile ions were removed from the eluent by the suppressor prior to detection, thus allowing a stable detector response, especially with the prototype electrolytic suppressor. The detector response for ten weakly anionic pharmaceuticals followed the expected models and the limits of detection obtained were not compromised by the use of a suppressor, yielding values below 50 ng/mL with MS, low to sub μg/mL levels with CAD and 220 μg/mL with ELSD (25 L injections). When coupled to MS and CAD, the prototype electrolytic suppressor showed percentage relative standard deviations (%RSDs) in peak areas of 0.42.5% on average, compared to the chemical suppressor which yielded 1.53 fold higher %RSD values for the test analytes. The prototype electrolytic suppressor also generally exhibited wider linear response ranges than the chemical suppressor.

Item Details

Item Type:Refereed Article
Keywords:suppressors, ion chromatography, pharmaceuticals, corona; CAD, ELSD, mass spectrometry
Research Division:Chemical Sciences
Research Group:Analytical Chemistry
Research Field:Separation Science
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Chemical Sciences
Author:Karu, N (Dr Naama Karu)
Author:Hutchinson, JP (Dr Joseph Hutchinson)
Author:Dicinoski, GW (Associate Professor Gregory Dicinoski)
Author:Haddad, PR (Professor Paul Haddad)
ID Code:82364
Year Published:2012
Web of Science® Times Cited:9
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2013-01-29
Last Modified:2013-04-22
Downloads:0

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