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The use of CFSE-like dyes for measuring lymphocyte proliferation: experimental considerations and biological variables

Citation

Quah, BJC and Lyons, AB and Parish, CR, The use of CFSE-like dyes for measuring lymphocyte proliferation: experimental considerations and biological variables, Mathematical Modelling of Natural Phenomena, 7, (5) pp. 53 - 64. ISSN 0973-5348 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 EDP Sciences

DOI: doi:10.1051/mmnp/20127505

Abstract

The measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.

Item Details

Item Type:Refereed Article
Keywords:Cell proliferation, CFSE, Flow Cytometry
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Lyons, AB (Dr Bruce Lyons)
ID Code:82338
Year Published:2012
Deposited By:Medicine (Discipline)
Deposited On:2013-01-25
Last Modified:2015-01-27
Downloads:0

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