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The use of CFSE-like dyes for measuring lymphocyte proliferation: experimental considerations and biological variables
journal contribution
posted on 2023-05-17, 15:37 authored by Quah, BJC, Alan Lyons, Parish, CRThe measurement of CFSE dilution by flow cytometry is a powerful experimental tool to measure lymphocyte proliferation. CFSE fluorescence precisely halves after each cell division in a highly predictable manner and is thus highly amenable to mathematical modelling. However, there are several biological and experimental conditions that can affect the quality of the proliferation data generated, which may be important to consider when modelling dye dilution data sets. Here we overview several of these variables including the type of fluorescent dye used to monitor cell division, dye labelling methodology, lymphocyte subset differences, in vitro versus in vivo experimental assays, cell autofluorescence, and dye transfer between cells.
History
Publication title
Mathematical Modelling of Natural PhenomenaVolume
7Issue
5Pagination
53 - 64ISSN
0973-5348Department/School
Tasmanian School of MedicinePublisher
EDP SciencesPlace of publication
17 Ave du Hoggar, Les Ulis, 91944 FranceRights statement
Copyright 2012 EDP SciencesRepository Status
- Restricted