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Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid

Citation

Casey, NP and Woods, GM, Anti-PML-RARα shRNA sensitises promyelocytic leukaemia cells to all-trans retinoic acid, Journal of RNAi and Gene Silencing, 8 pp. 464-469. ISSN 1747-0854 (2012) [Refereed Article]


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Licenced under Creative Commons Attribution-NonCommercial 2.5 Generic (CC BY-NC 2.5) http://creativecommons.org/licenses/by-nc/2.5/

Official URL: http://www.libpubmedia.co.uk/RNAiJ-Issues/Volume-8...

Abstract

The PML-RARα fusion gene disrupts the retinoic acid differentiation signal in a range of leukaemia types, promoting proliferation. We designed a shRNA to target the fusion mRNA, and the shRNA expression cassette was delivered via lentiviral transduction. Delivery of this shRNA significantly down regulated the target mRNA, with effects also evident at the protein level. When combined with ATRA administration, this down regulation of the fusion gene strongly inhibited proliferation in the NB4 PML cell line.

Item Details

Item Type:Refereed Article
Keywords:leukaemia, PML, shRNA, lentiviral vector
Research Division:Medical and Health Sciences
Research Group:Oncology and Carcinogenesis
Research Field:Cancer Therapy (excl. Chemotherapy and Radiation Therapy)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cancer and Related Disorders
Author:Casey, NP (Mr Nicholas Casey)
Author:Woods, GM (Professor Gregory Woods)
ID Code:82200
Year Published:2012
Deposited By:Menzies Institute for Medical Research
Deposited On:2013-01-21
Last Modified:2013-07-24
Downloads:217 View Download Statistics

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