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Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection


Durand, A and Chase, Z and Remenyi, TA and Queroue, F, Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection, Frontiers in Microbiology, 3 Article 437. ISSN 1664-302X (2013) [Refereed Article]


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Licensed under Creative Commons Attribution 3.0 Unported

DOI: doi:10.3389/fmicb.2012.00437


We have developed a method for the determination of copper in natural waters at nanomolar levels. Theuseofamicroplate-reader minimizes sample processing time(∼25s per sample), reagent consumption (∼120μL per sample),and sample volume(∼700μL). Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less than 7% on blanks and the detection limit is 0.7 nM for sea water and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique,which is simple,robust,and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist(e.g.,chemiluminescence,fluorescence,andabsorbance)could be adapted to the microplate-reader.

Item Details

Item Type:Refereed Article
Keywords:copper, microplate-reader, seawater, freshwater
Research Division:Earth Sciences
Research Group:Oceanography
Research Field:Chemical oceanography
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Measurement and assessment of estuarine water quality
UTAS Author:Durand, A (Mr Axel Durand)
UTAS Author:Chase, Z (Professor Zanna Chase)
UTAS Author:Remenyi, TA (Dr Tom Remenyi)
UTAS Author:Queroue, F (Mr Fabien Queroue)
ID Code:82176
Year Published:2013
Web of Science® Times Cited:14
Deposited By:IMAS Research and Education Centre
Deposited On:2013-01-17
Last Modified:2017-10-30
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