2013 Durand.pdf (1.33 MB)
Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection
journal contribution
posted on 2023-05-17, 15:28 authored by Axel DurandAxel Durand, Zanna ChaseZanna Chase, Tomas Remenyi, Queroue, FWe have developed a method for the determination of copper in natural waters at nanomolar levels. Theuseofamicroplate-reader minimizes sample processing time(∼25s per sample), reagent consumption (∼120μL per sample),and sample volume(∼700μL). Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less than 7% on blanks and the detection limit is 0.7 nM for sea water and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique,which is simple,robust,and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist(e.g.,chemiluminescence,fluorescence,andabsorbance)could be adapted to the microplate-reader.
History
Publication title
Frontiers in MicrobiologyArticle number
437Number
437Pagination
1-9ISSN
1664-302XDepartment/School
Institute for Marine and Antarctic StudiesPublisher
Frontiers Research FoundationPlace of publication
SwitzerlandRights statement
Licensed under Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/Repository Status
- Open