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Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection

Citation

Durand, A and Chase, Z and Remenyi, TA and Queroue, F, Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection, Frontiers in Microbiology, 3 Article 437. ISSN 1664-302X (2013) [Refereed Article]


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Licensed under Creative Commons Attribution 3.0 Unported http://creativecommons.org/licenses/by/3.0/

DOI: doi:10.3389/fmicb.2012.00437

Abstract

We have developed a method for the determination of copper in natural waters at nanomolar levels. Theuseofamicroplate-reader minimizes sample processing time(∼25s per sample), reagent consumption (∼120μL per sample),and sample volume(∼700μL). Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less than 7% on blanks and the detection limit is 0.7 nM for sea water and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique,which is simple,robust,and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist(e.g.,chemiluminescence,fluorescence,andabsorbance)could be adapted to the microplate-reader.

Item Details

Item Type:Refereed Article
Keywords:copper, microplate-reader, seawater, freshwater
Research Division:Earth Sciences
Research Group:Oceanography
Research Field:Chemical Oceanography
Objective Division:Environment
Objective Group:Physical and Chemical Conditions of Water
Objective Field:Physical and Chemical Conditions of Water in Coastal and Estuarine Environments
Author:Durand, A (Mr Axel Durand)
Author:Chase, Z (Associate Professor Zanna Chase)
Author:Remenyi, TA (Dr Tom Remenyi)
Author:Queroue, F (Mr Fabien Queroue)
ID Code:82176
Year Published:2013
Web of Science® Times Cited:5
Deposited By:IMAS Research and Education Centre
Deposited On:2013-01-17
Last Modified:2015-02-02
Downloads:223 View Download Statistics

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