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Calcium- and potassium-permeable plasma membrane transporters are activated by copper in Arabidopsis root tips: linking copper transport with cytosolic hydroxyl radical production

Citation

Rodrigo-Moreno, A and Andres-Colas, N and Poschenrieder, C and Gunse, B and Penarrubia, L and Shabala, S, Calcium- and potassium-permeable plasma membrane transporters are activated by copper in Arabidopsis root tips: linking copper transport with cytosolic hydroxyl radical production, Plant, Cell and Environment, 36, (4) pp. 844-855. ISSN 0140-7791 (2013) [Refereed Article]

Copyright Statement

Copyright 2012 Blackwell Publishing Ltd

DOI: doi:10.1111/pce.12020

Abstract

Transition metals such as copper can interact with ascorbate or hydrogen peroxide to form highly reactive hydroxyl radicals (OH.), with numerous implications to membrane transport activity and cell metabolism. So far, such interaction was described for extracellular (apoplastic) space but not cytosol. Here, a range of advanced electrophysiological and imaging techniques were applied to Arabidopsis thaliana plants differing in their copper-transport activity: Col-0, high-affinity copper transporter COPT1-overexpressing (C1OE) seedlings, and T-DNA COPT1 insertion mutant (copt1). Low Cu concentrations (10μm) stimulated a dose-dependent Gd3+ and verapamil sensitive net Ca2+ influx in the root apex but not in mature zone. C1OE also showed a fivefold higher Cu-induced K+ efflux at the root tip level compared with Col-0, and a reduction in basal peroxide accumulation at the root tip after copper exposure. Copper caused membrane disruptions of the root apex in C1OE seedlings but not in copt1 plants; this damage was prevented by pretreatment with Gd3+. Our results suggest that copper transport into cytosol in root apex results in hydroxyl radical generation at the cytosolic side, with a consequent regulation of plasma membrane OH.-sensitive Ca2+ and K+ transport systems.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis thaliana, Ca2+, COPT1, K+, oxidative stress, toxicity
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Shabala, S (Professor Sergey Shabala)
ID Code:82128
Year Published:2013
Web of Science® Times Cited:37
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-01-16
Last Modified:2014-04-04
Downloads:0

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