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Haem oxygenase modifies salinity tolerance in Arabidopsis by controlling K+ retention via regulation of the plasma membrane H+-ATPase and by altering SOS1 transcript levels in roots

Citation

Bose, J and Xie, Y and Shen, W and Shabala, S, Haem oxygenase modifies salinity tolerance in Arabidopsis by controlling K+ retention via regulation of the plasma membrane H+-ATPase and by altering SOS1 transcript levels in roots, Journal of Experimental Botany, 64, (2) pp. 471-481. ISSN 0022-0957 (2013) [Refereed Article]


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Licensed under Creative Commons Attribution-NonCommercial 2.0 UK: England & Wales(CC BY-NC 2.0 UK) http://creativecommons.org/licenses/by-nc/2.0/uk/

DOI: doi:10.1093/jxb/ers343

Abstract

Reactive oxygen species (ROS) production is a common denominator in a variety of biotic and abiotic stresses, including salinity. In recent years, haem oxygenase (HO; EC 1.14.99.3) has been described as an important component of the antioxidant defence system in both mammalian and plant systems. Moreover, a recent report on Arabidopsis demonstrated that HO overexpression resulted in an enhanced salinity tolerance in this species. However, physiological mechanisms and downstream targets responsible for the observed salinity tolerance in these HO mutants remain elusive. To address this gap, ion transport characteristics (K+ and H+ fluxes and membrane potentials) and gene expression profiles in the roots of Arabidopsis thaliana HO-overexpressing (35S:HY1-1/2/3/4) and loss-of-function (hy-100, ho2, ho3, and ho4) mutants were compared during salinity stress. Upon acute salt stress, HO-overexpressing mutants retained more K+ (less efflux), and exhibited better membrane potential regulation (maintained more negative potential) and higher H+ efflux activity in root epidermis, compared with loss-of-function mutants. Pharmacological experiments suggested that high activity of the plasma membrane H+-ATPase in HO overexpressor mutants provided the proton-motive force required for membrane potential maintenance and, hence, better K+ retention. The gene expression analysis after 12 h and 24 h of salt stress revealed high expression levels of H+-ATPases (AHA1/2/3)and Na+/H+ antiporter [salt overly sensitive1 (SOS1)] transcripts in the plasma membrane of HO overexpressors. It is concluded that HO modifies salinity tolerance in Arabidopsis by controlling K+ retention via regulation of the plasma membrane H+-ATPase and by altering SOS1 transcript levels in roots.

Item Details

Item Type:Refereed Article
Keywords:gene expression, H+-ATPase, haem oxygenase, ion fluxes, membrane potential, potassium, sodium
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Environmentally Sustainable Plant Production
Objective Field:Environmentally Sustainable Plant Production not elsewhere classified
Author:Bose, J (Dr Jayakumar Bose)
Author:Shabala, S (Professor Sergey Shabala)
ID Code:82123
Year Published:2013
Web of Science® Times Cited:31
Deposited By:Tasmanian Institute of Agriculture
Deposited On:2013-01-16
Last Modified:2015-02-04
Downloads:280 View Download Statistics

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