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Exposure of colonic epithelial cells to oxidative and endoplasmic reticulum stress causes rapid potassium efflux and calcium influx
Citation
Shabala, L and Walker, EJ and Eklund, A and Randall-Demllo, S and Shabala, S and Guven, N and Cook, AL and Eri, RD, Exposure of colonic epithelial cells to oxidative and endoplasmic reticulum stress causes rapid potassium efflux and calcium influx, Cell Biochemistry and Function, 31, (7) pp. 603-611. ISSN 1099-0844 (2013) [Refereed Article]
Copyright Statement
Copyright 2012 John Wiley & Sons, Ltd.
DOI: doi:10.1002/cbf.2946
Abstract
Endoplasmic reticulum (ER) stress and oxidative stress have recently been linked to the pathogenesis of inflammatory bowel diseases. Under physiological conditions, intestinal epithelial cells are exposed to ER and oxidative stress affecting the cellular ionic homeostasis. However, these altered ion flux ‘signatures’ during these stress conditions are poorly characterized. We investigated the kinetics of K+, Ca2+ and H+ ion fluxes during ER and oxidative stress in a colonic epithelial cell line LS174T using a non-invasive microelectrode ion flux estimation technique. ER and oxidative stress were induced by cell exposure to tunicamycin (TM) and copper ascorbate (CuAsc), respectively, from 1 to 24 h. Dramatic K+ efflux was observed following acute ER stress with peak K+ efflux being 306 and 1387 nmolm-2s-1 for 10
and 50μgml-1, respectively (p<0.01). TM-dependent Ca2+ uptake was more prolonged with peak values of 085 and 268 nmolm-2s-1 for 10 and 50 mgml-1 TM, respectively (p<0.02). Ion homeostasis was also affected by the duration of ER stress. Increased duration of TM treatment from 0 to 18 h led to increases in both K+ efflux and Ca2+ uptake. While K+ changes were significantly higher at each time point tested, Ca2+ uptake was significantly higher only after prolonged treatment (18 h). CuAsc also led to an increased K+ efflux
and Ca2+ uptake. Functional assays to investigate the effect of inhibiting K+ efflux with tetraethylammonium resulted in increased cell viability. We conclude that ER/oxidative stress in colonic epithelial cells cause dramatic K+, Ca2+ and H+ ion flux changes, which may predispose this lineage to poor stress recovery reminiscent of that seen in inflammatory bowel diseases.
Item Details
Item Type: | Refereed Article |
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Keywords: | ER stress, inflammatory bowel disease, ion flux, oxidative stress, colonic epithelial cells |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical biochemistry and metabolomics |
Research Field: | Medical biochemistry and metabolomics not elsewhere classified |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the health sciences |
UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
UTAS Author: | Walker, EJ (Mrs Emma Walker) |
UTAS Author: | Eklund, A (Miss Anne Eklund) |
UTAS Author: | Randall-Demllo, S (Mr Sarron Randall-Demllo) |
UTAS Author: | Shabala, S (Professor Sergey Shabala) |
UTAS Author: | Guven, N (Dr Nuri Guven) |
UTAS Author: | Cook, AL (Associate Professor Tony Cook) |
UTAS Author: | Eri, RD (Associate Professor Raj Eri) |
ID Code: | 81846 |
Year Published: | 2013 (online first 2012) |
Web of Science® Times Cited: | 7 |
Deposited By: | Pharmacy |
Deposited On: | 2013-01-09 |
Last Modified: | 2017-11-06 |
Downloads: | 0 |
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