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TRFLP analysis reveals that fungi rather than bacteria are associated with premature yeast flocculation in brewing


Kaur, M and Bowman, JP and Stewart, DC and Sheehy, M and Janusz, A and Speers, RA and Koutoulis, A and Evans, E, TRFLP analysis reveals that fungi rather than bacteria are associated with premature yeast flocculation in brewing, Journal of Industrial Microbiology and Biotechnology, 39, (12) pp. 1821-1832. ISSN 1367-5435 (2012) [Refereed Article]

Copyright Statement

Copyright Society for Industrial Microbiology and Biotechnology 2012

DOI: doi:10.1007/s10295-012-1188-8


Premature yeast flocculation (PYF) is a sporadic fermentation problem in the brewing industry that results in incomplete yeast utilization of fermentable sugars in wort. Culture-independent, PCR-based fingerprinting techniques were applied in this study to identify the associations between the occurrence of the PYF problem during brewery fermentation with barley malt-associated microbial communities (both bacteria and fungi). Striking differences in the microbial DNA fingerprint patterns for fungi between PYF positive (PYF +ve) and negative (PYF -ve) barley malts were observed using the terminal restriction fragment length polymorphism (TRFLP) technique. The presence of terminal restriction fragments (TRFs) of 360460 bp size range, for fungal HaeIII restriction enzyme-derived TRFLP profiles appeared to vary substantially between PYF +ve and PYF -ve samples. The source of the barley malt did not influence the fungal taxa implicated in PYF. TRFLP analysis indicates bacterial taxa are unlikely to be important in causing PYF. Virtual digestion of fungal sequences tentatively linked HaeIII TRFs in the 360460 bp size range to a diverse range of yeast/yeast-like species. Findings from this study suggest that direct monitoring of barley malt samples using molecular methods could potentially be an efficient and viable alternative for monitoring PYF during brewery fermentations.

Item Details

Item Type:Refereed Article
Keywords:barley malt, brewery fermentation, community fingerprinting, microbial communities, yeast
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture biochemistry and physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Kaur, M (Dr Mandeep Kaur)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
UTAS Author:Koutoulis, A (Professor Anthony Koutoulis)
UTAS Author:Evans, E (Dr Evan Evans)
ID Code:81561
Year Published:2012
Funding Support:Australian Research Council (LP0560329)
Web of Science® Times Cited:7
Deposited By:Agricultural Science
Deposited On:2012-12-14
Last Modified:2013-05-14
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