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TRFLP analysis reveals that fungi rather than bacteria are associated with premature yeast flocculation in brewing

journal contribution
posted on 2023-05-17, 15:02 authored by Mandeep KaurMandeep Kaur, John BowmanJohn Bowman, Stewart, DC, Sheehy, M, Janusz, A, Speers, RA, Anthony KoutoulisAnthony Koutoulis, Evans, E
Premature yeast flocculation (PYF) is a sporadic fermentation problem in the brewing industry that results in incomplete yeast utilization of fermentable sugars in wort. Culture-independent, PCR-based fingerprinting techniques were applied in this study to identify the associations between the occurrence of the PYF problem during brewery fermentation with barley malt-associated microbial communities (both bacteria and fungi). Striking differences in the microbial DNA fingerprint patterns for fungi between PYF positive (PYF +ve) and negative (PYF -ve) barley malts were observed using the terminal restriction fragment length polymorphism (TRFLP) technique. The presence of terminal restriction fragments (TRFs) of 360–460 bp size range, for fungal HaeIII restriction enzyme-derived TRFLP profiles appeared to vary substantially between PYF +ve and PYF -ve samples. The source of the barley malt did not influence the fungal taxa implicated in PYF. TRFLP analysis indicates bacterial taxa are unlikely to be important in causing PYF. Virtual digestion of fungal sequences tentatively linked HaeIII TRFs in the 360–460 bp size range to a diverse range of yeast/yeast-like species. Findings from this study suggest that direct monitoring of barley malt samples using molecular methods could potentially be an efficient and viable alternative for monitoring PYF during brewery fermentations.

Funding

Australian Research Council

AusMalt Pty Ltd

History

Publication title

Journal of Industrial Microbiology and Biotechnology

Volume

39

Issue

12

Pagination

1821-1832

ISSN

1367-5435

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Springer-Verlag Heidelberg

Place of publication

Tiergartenstrasse 17, Heidelberg, Germany, D-69121

Rights statement

Copyright Society for Industrial Microbiology and Biotechnology 2012

Repository Status

  • Restricted

Socio-economic Objectives

Barley

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