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Changes in the microbial community structure of bacteria, archaea and fungi in response to elevated CO2 and warming in an Australian native grassland soil

Citation

Hayden, HL and Mele, PM and Bougoure, DS and Allan, CY and Norng, S and Piceno, YM and Brodie, EL and DeSantis, TZ and Andersen, GL and Williams, AL and Hovenden, MJ, Changes in the microbial community structure of bacteria, archaea and fungi in response to elevated CO2 and warming in an Australian native grassland soil, Environmental Microbiology, 14, (12) pp. 3081-3096. ISSN 1462-2912 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Society for Applied Microbiology and Blackwell Publishing Ltd

DOI: doi:10.1111/j.1462-2920.2012.02855.x

Abstract

The microbial community structure of bacteria, archaea and fungi is described in an Australian native grassland soil after more than 5 years exposure to different atmospheric CO2 concentrations ([CO2]) (ambient, + 550 ppm) and temperatures (ambient, + 2C) under different plant functional types (C3 and C4 grasses) and at two soil depths (05 cm and 510 cm). Archaeal community diversity was influenced by elevated [CO2], while under warming archaeal 16S rRNA gene copy numbers increased for C4 plant Themeda triandra and decreased for the C3 plant community (P < 0.05). Fungal community diversity resulted in three groups based upon elevated [CO2], elevated [CO2] plus warming and ambient [CO2]. Overall bacterial community diversity was influenced primarily by depth. Specific bacterial taxa changed in richness and relative abundance in response to climate change factors when assessed by a high-resolution 16S rRNA microarray (Phylo- Chip). Operational taxonomic unit signal intensities increased under elevated [CO2] for both Firmicutes and Bacteroidetes, and increased under warming for Actinobacteria and Alphaproteobacteria. For the interaction of elevated [CO2] and warming there were 103 significant operational taxonomic units (P < 0.01) representing 15 phyla and 30 classes. The majority of these operational taxonomic units increased in abundance for elevated [CO2] plus warming plots, while abundance declined in warmed or elevated [CO2] plots. Bacterial abundance (16S rRNA gene copy number) was significantly different for the interaction of elevated [CO2] and depth (P < 0.05) with decreased abundance under elevated [CO2] at 510 cm, and for Firmicutes under elevated [CO2] (P < 0.05). Bacteria, archaea and fungi in soil responded differently to elevated [CO2], warming and their interaction. Taxa identified as significantly climate-responsive could show differing trends in the direction of response (+ or -) under elevated CO2 or warming, which could then not be used to predict their interactive effects supporting the need to investigate interactive effects for climate change. The approach of focusing on specific taxonomic groups provides greater potential for understanding complex microbial community changes in ecosystems under climate change.

Item Details

Item Type:Refereed Article
Keywords:elevated CO2, FACE, warming, microbial community function, fingerprinting, ecosystem function, nutrient cycling
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Terrestrial Ecology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Williams, AL (Ms Amanda Williams)
Author:Hovenden, MJ (Associate Professor Mark Hovenden)
ID Code:81536
Year Published:2012
Funding Support:Australian Research Council (DP0984779)
Web of Science® Times Cited:37
Deposited By:Plant Science
Deposited On:2012-12-12
Last Modified:2017-11-03
Downloads:0

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