eCite Digital Repository
Controlled ultraviolet (UV) photoinitiated fabrication of monolithic porous layer open tubular (monoPLOT) capillary columns for chromatographic applications
Citation
Collins, DA and Nesterenko, EP and Brabazon, D and Paull, B, Controlled ultraviolet (UV) photoinitiated fabrication of monolithic porous layer open tubular (monoPLOT) capillary columns for chromatographic applications, Analytical Chemistry, 84, (7) pp. 3465-3472. ISSN 0003-2700 (2012) [Refereed Article]
Copyright Statement
Copyright 2012 American Chemical Society
Abstract
An automated column fabrication technique that is based on a ultraviolet (UV) light-emitting diode (LED) array oven, and provides precisely controlled "in-capillary" ultraviolet (UV) initiated polymerization at 365 nm, is presented for the production of open tubular monolithic porous polymer layer capillary (monoPLOT) columns of varying length, inner diameter (ID), and porous layer thickness. The developed approach allows the preparation of columns of varying length, because of an automated capillary delivery approach, with precisely controlled and uniform layer thickness and monolith morphology, from controlled UV power and exposure time. The relationships between direct exposure times, intensity, and layer thickness were determined, as were the effects of capillary delivery rate (indirect exposure rate), and multiple exposures on the layer thickness and axial distribution. Layer thickness measurements were taken by scanning electron microscopy (SEM), with the longitudinal homogeneity of the stationary phase confirmed using scanning capacitively coupled contactless conductivity detection (sC4D). The new automated UV polymerization technique presented in this work allows the fabrication of monoPLOT columns with a very high column-to-column production reproducibility, displaying a longitudinal phase thickness variation within ±0.8% RSD (relative standard deviation).
Item Details
Item Type: | Refereed Article |
---|---|
Keywords: | automation, fabrication, polymerization, scanning electron microscopy, thickness measurement, light emitting diodes |
Research Division: | Chemical Sciences |
Research Group: | Analytical chemistry |
Research Field: | Separation science |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the chemical sciences |
UTAS Author: | Paull, B (Professor Brett Paull) |
ID Code: | 81030 |
Year Published: | 2012 |
Web of Science® Times Cited: | 38 |
Deposited By: | Austn Centre for Research in Separation Science |
Deposited On: | 2012-11-21 |
Last Modified: | 2013-05-07 |
Downloads: | 0 |
Repository Staff Only: item control page