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The reproducibility of 31-phosphorous MRS measures of muscle energetics at 3 tesla in trained men

Citation

Edwards, LM and Tyler, DJ and Kemp, GJ and Dwyer, RM and Johnson, A and Holloway, CJ and Nevill, AM and Clarke, K, The reproducibility of 31-phosphorous MRS measures of muscle energetics at 3 tesla in trained men, PLoS One, 7, (6) Article e37237. ISSN 1932-6203 (2012) [Refereed Article]


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Licensed under Creative Commons Attribution 2.5 Generic (CC BY 2.5) http://creativecommons.org/licenses/by/2.5/

DOI: doi:10.1371/journal.pone.0037237

Abstract

Objective Magnetic resonance spectroscopy (MRS) provides an exceptional opportunity for the study of in vivo metabolism. MRS is widely used to measure phosphorus metabolites in trained muscle, although there are no published data regarding its reproducibility in this specialized cohort. Thus, the aim of this study was to assess the reproducibility of 31P-MRS in trained skeletal muscle. Methods We recruited fifteen trained men (VO2peak = 4.70.8 L min−1/588 mL kg−1 min−1) and performed duplicate MR experiments during plantar flexion exercise, three weeks apart. Results Measures of resting phosphorus metabolites were reproducible, with 1.7 mM the smallest detectable difference in phosphocreatine (PCr). Measures of metabolites during exercise were less reliable: exercising PCr had a coefficient of variation (CV) of 27% during exercise, compared with 8% at rest. Estimates of mitochondrial function were variable, but experimentally useful. The CV of PCr1/2t was 40%, yet much of this variance was inter-subject such that differences of <20% were detectable with n = 15, given a significance threshold of p<0.05. Conclusions 31-phosphorus MRS provides reproducible and experimentally useful measures of phosphorus metabolites and mitochondrial function in trained human skeletal muscle.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Physiology
Research Field:Physiology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Medical and Health Sciences
Author:Dwyer, RM (Dr Renee Dwyer)
ID Code:80995
Year Published:2012
Web of Science® Times Cited:14
Deposited By:Medicine (Discipline)
Deposited On:2012-11-20
Last Modified:2017-11-07
Downloads:171 View Download Statistics

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