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Epoxy-based monoliths for capillary liquid chromatography of small and large molecules


Talebi, M and Arrua, RD and Gaspar, A and Lacher, NA and Wang, Q and Haddad, PR and Hilder, EF, Epoxy-based monoliths for capillary liquid chromatography of small and large molecules, Analytical and Bioanalytical Chemistry, 405, (7) pp. 2233-2244. ISSN 1618-2642 (2013) [Refereed Article]

Copyright Statement

Copyright 2012 Springer-Verlag

DOI: doi:10.1007/s00216-012-6486-5


A versatile epoxy-based monolith was synthesised by polycondensation polymerisation of glycidyl ether 100 with ethylenediamine using a porogenic system consisting of polyethylene glycol, Mw = 1000, and 1-decanol. Polymerisation was performed at 80C for 22h. A simple acid hydrolysis of residual epoxides resulted in a mixed diol-amino chemistry. The modified column was used successfully for hydrophilic interaction liquid chromatography (HILIC) of small molecule probes such as nucleic acid bases and nucleosides, benzoic acid derivatives, as well as for peptides released from a tryptic digest of cytochrome c. The mixed-mode chemistry allowed both hydrophilic partitioning and ion-exchange (IEX) interactions to contribute to the separation, providing flexibility in selectivity control. Residual epoxide groups were also exploited for incorporating a mixed IEX chemistry. Alternatively, the surface chemistry of the monolith pore surface rendered hydrophobic via grafting of a co-polymerised hydrophobic hydrogel. The inherent hydrophilicity of the monolith scaffold also enabled high performance separation of proteins under IEX and hydrophobic interaction modes and in the absence of non-specific interactions.

Item Details

Item Type:Refereed Article
Keywords:epoxy-based monolith, hydrophilic interaction chromatography (HILIC), capillary liquid chromatography Proteins. Peptides. Liquid chromatography-mass spectrometry (LC-MS)
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Talebi, M (Dr Mohammad Talebi)
UTAS Author:Arrua, RD (Dr Dario Arrua)
UTAS Author:Gaspar, A (Dr Andras Gaspar)
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Hilder, EF (Professor Emily Hilder)
ID Code:80941
Year Published:2013
Web of Science® Times Cited:16
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2012-11-19
Last Modified:2017-10-29

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