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Review of recent advances in the preparation of organic polymer monoliths for liquid chromatography of large molecules


Arrua, RD and Talebi, M and Causon, TJ and Hilder, EF, Review of recent advances in the preparation of organic polymer monoliths for liquid chromatography of large molecules, Analytica Chimica Acta, 738 pp. 1-12. ISSN 0003-2670 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Elsevier B.V.

DOI: doi:10.1016/j.aca.2012.05.052


In recent years the use of monolithic polymers in separation science has greatly increased due to the advantages these materials present over particle-based stationary phases, such as their relative ease of preparation and good permeability. For these reasons, these materials present high potential as stationary phases for the separation and purification of large molecules such as proteins, peptides, nucleic acids and cells. An example of this is the wide range of commercial available polymer-based monolithic columns now present in the market.

This review summarizes recent developments in the synthesis of monolithic polymers for separation science, such as the incorporation of nanostructures in the polymeric scaffold as well as the preparation of hybrid structures. The different methods used in the surface functionalization of monolithic columns are also reviewed. Finally, we critically discuss the recent applications of this column technology in the separation of large molecules under different chromatographic mode.

Item Details

Item Type:Refereed Article
Keywords:polymer monoliths, ion-exchange, reversed phase, proteins, liquid chromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Arrua, RD (Dr Dario Arrua)
UTAS Author:Talebi, M (Dr Mohammad Talebi)
UTAS Author:Causon, TJ (Mr Timothy Causon)
UTAS Author:Hilder, EF (Professor Emily Hilder)
ID Code:80940
Year Published:2012
Web of Science® Times Cited:114
Deposited By:Austn Centre for Research in Separation Science
Deposited On:2012-11-19
Last Modified:2015-02-07

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