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Multiplex PCR assay for the detection of Pantoea stewartii subsp. stewartii using species-specific genetic markers

Citation

Thapa, SP and Park, DH and Wilson, C and Hur, JH and Lim, CK, Multiplex PCR assay for the detection of Pantoea stewartii subsp. stewartii using species-specific genetic markers, Australasian Plant Pathology, 41, (5) pp. 559-564. ISSN 0815-3191 (2012) [Refereed Article]

Copyright Statement

Copyright 2012 Australasian Plant Pathology Society Inc.

DOI: doi:10.1007/s13313-012-0123-9

Abstract

We developed a multiplex PCR detection assay for quick diagnosis of Pantoea stewartii subsp. stewartii the causal agent of Stewart’s bacterial wilt and leaf blight of maize, using specific genetic markers. The genetic markers of wtsE, cpsA and hrpN gene specifically amplified 519 bp, 375 bp and 200 bp of DNA, respectively, from all isolates of P. stewartii subsp. stewartii but not from other phytopathogenic bacteria. The sensitivity of the genetic markers is down to 103 CFU/mL (5 cells per reaction) of bacterial culture and ~0.5 pg of genomic DNA. In sampling studies, the assay detected P. stewartii subsp. stewartii from artificially infected maize plants 12 days post inoculation. The PCR based genetic markers are specific and they give selective and sensitive amplification of P. stewartii subsp. stewartii for diagnosis in mixed cultures and in planta.

Item Details

Item Type:Refereed Article
Keywords:genetic marker, multiplex PCR, P. stewartii subsp. stewartii, specific detection
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Crop and Pasture Protection (Pests, Diseases and Weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Summer Grains and Oilseeds
Objective Field:Maize
Author:Wilson, C (Associate Professor Calum Wilson)
ID Code:80579
Year Published:2012
Web of Science® Times Cited:5
Deposited By:Agricultural Science
Deposited On:2012-11-02
Last Modified:2015-06-25
Downloads:1 View Download Statistics

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